Anti-FEN1 antibody (50-380) (STJ23646)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal FEN1 (50-380) antibody for use in WB, IHC-P, IF, ICC, IP and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/IP/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IHC-P:1:50-1:200IF/ICC:1:10-1:100IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specif |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | FEN1 |
| Gene ID | 2237 |
| Uniprot ID | FEN1_HUMAN |
| Immunogen Region | 50-380 |
| Immunogen Sequence | GDVLQNEEGETTSHLMGMFY RTIRMMENGIKPVYVFDGKP PQLKSGELAKRSERRAEAEK QLQQAQAAGAEQEVEKFTKR LVKVTKQHNDECKHLLSLMG IPYLDAPSEAEASCAALVKA GKVYAAATEDMDCLTFGSPV LMRHLTASEAKKLPIQEFHL SRILQELGLNQEQFVDLCIL LGSDYCESIRGIGPKRAVDL IQKHKSIEEIVRRLDPNKYP VPENWLHKEAHQLFLEPEV |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 50-380 of human FEN-1 (NP_004102.1). |
Additional Info
| Post Translational Modifications | Acetylated by EP300. Acetylation inhibits both endonuclease and exonuclease activity. Acetylation also reduces DNA-binding activity but does not affect interaction with PCNA or EP300. Phosphorylation upon DNA damage induces relocalization to the nuclear plasma. Phosphorylation at Ser-187 by CDK2 occurs during late S-phase and results in dissociation from PCNA. Methylation at Arg-192 by PRMT5 impedes Ser-187 phosphorylation and increases interaction with PCNA. |
| Function | Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structures that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA. |
| Protein Name | Flap Endonuclease 1Fen-1Dnase IvFlap Structure-Specific Endonuclease 1Maturation Factor 1Mf1Hfen-1 |
| Database Links | Reactome: R-HSA-110362Reactome: R-HSA-162594Reactome: R-HSA-174437Reactome: R-HSA-5651801Reactome: R-HSA-5685939Reactome: R-HSA-69166 |
| Cellular Localisation | Isoform 1: NucleusNucleolusNucleusNucleoplasmResides Mostly In The Nucleoli And Relocalizes To The Nucleoplasm Upon Dna DamageIsoform Fenmit: Mitochondrion |
| Alternative Antibody Names | Anti-Flap Endonuclease 1 antibodyAnti-Fen-1 antibodyAnti-Dnase Iv antibodyAnti-Flap Structure-Specific Endonuclease 1 antibodyAnti-Maturation Factor 1 antibodyAnti-Mf1 antibodyAnti-Hfen-1 antibodyAnti-FEN1 antibodyAnti-RAD2 antibody |
Information sourced from Uniprot.org