• Western blot analysis of extracts of various cell lines, using FCRL3 antibody (STJ112480) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-FCRL3 antibody (600-720) (STJ112480)

SKU:
STJ112480

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-FCRL3 (600-720) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: FCRL3
Gene ID: 115352
Uniprot ID: FCRL3_HUMAN
Immunogen Region: 600-720
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 600-720 of human FCRL3 (NP_443171.2).
Immunogen Sequence: RRKPGGLSATGTSSHSPSEC QEPSSSRPSRIDPQEPTHSK PLAPMELEPMYSNVNPGDSN PIYSQIWSIQHTKENSANCP MMHQEHEELTVLYSELKKTH PDDSAGEASSRGRAHEEDDE E
Tissue Specificity Primarily expressed in secondary lymphoid tissues by mature subsets of B-cells. Low expression on transitional B cells which increases to higher surface expression on mature and memory B-cells with innate-like features (at protein level). Expressed a low levels in naive and germinal center B-cells but also expressed in NK cells (at protein level). Expressed in unfertilized oocytes (at protein level). Expressed in a population of thymically derived naturally occurring regulatory T-cells that exhibits a memory phenotype, specialized in suppressing immune response to self-antigens. Detected in spleen, lymph node, peripheral blood lymphocytes, thymus, bone marrow, kidney, salivary gland, adrenal gland and uterus.
Post Translational Modifications Phosphorylated on cytoplasmic tyrosines.required for interaction with protein tyrosine phosphatases and protein tyrosine kinases.
Function Promotes TLR9-induced B-cell proliferation, activation and survival but inhibits antibody production and suppresses plasma cell differentiation. Enhances activation of NF-kappa-B and MAPK signaling pathways in TLR9 stimulated B-cells. Has inhibitory potentional on B-cell receptor (BCR)-mediated signaling, possibly through association with SH2 domain-containing phosphatases. Inhibits cell tyrosine phosphorylation, calcium mobilization and activation-induced cell death induced through BCR signaling. Regulatory T-cells expressing FCRL3 exhibit a memory phenotype, are relatively nonresponsive to antigenic stimulation in presence of IL2 and have reduced capacity to suppress the proliferation of effector T-cells. Acts as a human-specific epitope on the cell surface of oocytes (oolemma) and plays a role during sperm-egg adhesion and fusion. Interacts with the IZUMO1-IZUMO1R/JUNO sperm-egg complex and replaces IZUMO1R/JUNO as IZUMO1 receptor during fertilization, thereby permitting species-specific gamete fusion.
Protein Name Fc Receptor-Like Protein 3
Fcr-Like Protein 3
Fcrl3
Fc Receptor Homolog 3
Fcrh3
Ifgp Family Protein 3
Hifgp3
Immune Receptor Translocation-Associated Protein 3
Maia
Sh2 Domain-Containing Phosphatase Anchor Protein 2
Cd Antigen Cd307c
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Cell Projection
Microvillus Membrane
Localized Along The Oolemma Microvilli Of Unfertilized Oocytes
Alternative Antibody Names Anti-Fc Receptor-Like Protein 3 antibody
Anti-Fcr-Like Protein 3 antibody
Anti-Fcrl3 antibody
Anti-Fc Receptor Homolog 3 antibody
Anti-Fcrh3 antibody
Anti-Ifgp Family Protein 3 antibody
Anti-Hifgp3 antibody
Anti-Immune Receptor Translocation-Associated Protein 3 antibody
Anti-Maia antibody
Anti-Sh2 Domain-Containing Phosphatase Anchor Protein 2 antibody
Anti-Cd Antigen Cd307c antibody
Anti-FCRL3 antibody
Anti-FCRH3 antibody
Anti-IFGP3 antibody
Anti-IRTA3 antibody
Anti-SPAP2 antibody

Information sourced from Uniprot.org

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