• Immunohistochemistry analysis of FCHSD1 in paraffin-embedded Mouse kidney using FCHSD1 Rabbit polyclonal antibody (STJ117738) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of FCHSD1 in paraffin-embedded Rat spleen using FCHSD1 Rabbit polyclonal antibody (STJ117738) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using FCHSD1 Rabbit polyclonal antibody (STJ117738) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15S.

Anti-FCHSD1 antibody (100-400) (STJ117738)

SKU:
STJ117738

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-FCHSD1 (100-400) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:200-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: FCHSD1
Gene ID: 89848
Uniprot ID: FCSD1_HUMAN
Immunogen Region: 100-400
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 100-400 of human FCHSD1 (NP_258260.1).
Immunogen Sequence: RLQASDRYRDLAGGTGRSAK EQVLRKGTENLQRAQAEVLQ SVRELSRSRKLYGQRERVWA LAQEKAADVQARLNRSDHGI FHSRTSLQKLSTKLSAQSAQ YSQQLQAARNEYLLNLVATN AHLDHYYQEELPALLKALVS ELSEHLRDPLTSLSHTELEA AEVILEHAHRGEQTTSQVSW EQDLKLFLQEPGVFSPTPPQ QFQPAGTDQVCVLEWGAEGV AGKSGLEKEVQRLTSRAAR
Function Promotes actin polymerization mediated by SNX9 and WASL.
Protein Name F-Bar And Double Sh3 Domains Protein 1
Protein Nervous Wreck 2
Nwk2
Cellular Localisation Cytoplasm
Perikaryon
Cell Projection
Cytoplasmic Vesicle
Detected On Neuronal Cell Bodies And Cell Projections
In Part On Cytoplasmic Vesicles
Alternative Antibody Names Anti-F-Bar And Double Sh3 Domains Protein 1 antibody
Anti-Protein Nervous Wreck 2 antibody
Anti-Nwk2 antibody
Anti-FCHSD1 antibody
Anti-UNQ737 antibody
Anti-PRO1431 antibody

Information sourced from Uniprot.org

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