Anti-FCHO2 antibody (1-300) (STJ117155)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal FCHO2 (1-300) antibody for use in WB and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | FCHO2 |
| Gene ID | 115548 |
| Uniprot ID | FCHO2_HUMAN |
| Immunogen Region | 1-300 |
| Immunogen Sequence | MVMAYFVENFWGEKNSGFDV LYHNMKHGQISTKELADFVR ERATIEEAYSRSMTKLAKSA SNYSQLGTFAPVWDVFKTST EKLANCHLDLVRKLQELIKE VQKYGEEQVKSHKKTKEEVA GTLEAVQTIQSITQALQKSK ENYNAKCVEQERLKKEGATQ REIEKAAVKSKKATDTYKLY VEKYALAKADFEQKMTETAQ KFQDIEETHLIHIKEIIGSL SNAIKEIHLQIGQVHEEFI |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human FCHO2 (NP_620137.2). |
Additional Info
| Post Translational Modifications | Ubiquitinated. Mainly undergoes monoubiquitination but also polyubiquitination. |
| Function | Functions in an early step of clathrin-mediated endocytosis. Has both a membrane binding/bending activity and the ability to recruit proteins essential to the formation of functional clathrin-coated pits. Has a lipid-binding activity with a preference for membranes enriched in phosphatidylserine and phosphoinositides (Pi(4,5) biphosphate) like the plasma membrane. Its membrane-bending activity might be important for the subsequent action of clathrin and adaptors in the formation of clathrin-coated vesicles. Involved in adaptor protein complex AP-2-dependent endocytosis of the transferrin receptor, it also functions in the AP-2-independent endocytosis of the LDL receptor. |
| Protein Name | F-Bar Domain Only Protein 2 |
| Database Links | Reactome: R-HSA-8856825Reactome: R-HSA-8856828 |
| Cellular Localisation | MembraneClathrin-Coated PitPeripheral Membrane ProteinCytoplasmic SideAssociated With Forming But Not Mature Clathrin-Coated VesiclesThe Recruitment To Coated-Pits Precede The One Of Clathrin And The Adaptor Protein Complex Ap-2 |
| Alternative Antibody Names | Anti-F-Bar Domain Only Protein 2 antibodyAnti-FCHO2 antibody |
Information sourced from Uniprot.org