• Immunofluorescence analysis of U-2 OS cells using FCF1 Rabbit polyclonal antibody (STJ119324) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using FCF1 Rabbit polyclonal antibody (STJ119324) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of FCF1 in paraffin-embedded Human esophageal using FCF1 Rabbit polyclonal antibody (STJ119324) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from 293T cells, using FCF1 Rabbit polyclonal antibody (STJ119324) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 100s.

Anti-FCF1 antibody (100-198) (STJ119324)

SKU:
STJ119324

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-FCF1 (100-198) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: FCF1
Gene ID: 51077
Uniprot ID: FCF1_HUMAN
Immunogen Region: 100-198
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 100-198 of human FCF1 (NP_057046.1).
Immunogen Sequence: PCITDCVMAEIEKLGQKYRV ALRIAKDPRFERLPCTHKGT YADDCLVQRVTQHKCYIVAT VDRDLKRRIRKIPGVPIMYI SNHRYNIERMPDDYGAPRF
Function Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome.
Protein Name Rrna-Processing Protein Fcf1 Homolog
Database Links Reactome: R-HSA-6790901
Reactome: R-HSA-6791226
Cellular Localisation Nucleus
Nucleolus
Alternative Antibody Names Anti-Rrna-Processing Protein Fcf1 Homolog antibody
Anti-FCF1 antibody
Anti-C14orf111 antibody
Anti-CGI-35 antibody

Information sourced from Uniprot.org

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