Anti-FBXW7 antibody (STJ11108359)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthetic peptide. This information is considered to be commercially sensitive.
General Information
| Short Description | Rabbit polyclonal F-Box/Wd Repeat-Containing Protein 7 antibody for use in WB, IHC-P and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB, 1:500-1:1000IHC-P, 1:50-1:200ELISA, Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS containing 50% Glycerol, preserved with proclin300 or Sodium Azide (as specified on the Certificate of Analysis) , pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | FBXW7 |
| Gene ID | 55294 |
| Uniprot ID | FBXW7_HUMAN |
| Immunogen | Synthetic peptide. This information is considered to be commercially sensitive. |
| Immunogen Sequence | Human |
Additional Info
| Post Translational Modifications | Phosphorylation at Thr-205 promotes interaction with PIN1, leading to disrupt FBXW7 dimerization and promoting FBXW7 autoubiquitination and degradation. Phosphorylated by ATM at Ser-26 in response to DNA damage, promoting recruitment to DNA damage sites and 'Lys-63'-linked ubiquitination of phosphorylated XRCC4. Ubiquitinated: autoubiquitinates following phosphorylation at Thr-205 and subsequent interaction with PIN1. Ubiquitination leads to its proteasomal degradation. |
| Function | Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Recognizes and binds phosphorylated sites/phosphodegrons within target proteins and thereafter brings them to the SCF complex for ubiquitination. Identified substrates include cyclin-E (CCNE1 or CCNE2), DISC1, JUN, MYC, NOTCH1 released notch intracellular domain (NICD), NFE2L1, NOTCH2, MCL1, MLST8, RICTOR, and probably PSEN1. Acts as a negative regulator of JNK signaling by binding to phosphorylated JUN and promoting its ubiquitination and subsequent degradation. Involved in bone homeostasis and negative regulation of osteoclast differentiation. Regulates the amplitude of the cyclic expression of hepatic core clock genes and genes involved in lipid and glucose metabolism via ubiquitination and proteasomal degradation of their transcriptional repressor NR1D1.CDK1-dependent phosphorylation of NR1D1 is necessary for SCF(FBXW7)-mediated ubiquitination. Also able to promote 'Lys-63'-linked ubiquitination in response to DNA damage. The SCF(FBXW7) complex facilitates double-strand break repair following phosphorylation by ATM: phosphorylation promotes localization to sites of double-strand breaks and 'Lys-63'-linked ubiquitination of phosphorylated XRCC4, enhancing DNA non-homologous end joining. |
| Protein Name | F-Box/Wd Repeat-Containing Protein 7Archipelago HomologHagoF-Box And Wd-40 Domain-Containing Protein 7F-Box Protein Fbx30Sel-10Hcdc4 |
| Database Links | Reactome: R-HSA-2122947Reactome: R-HSA-2644606Reactome: R-HSA-2644607Reactome: R-HSA-2894862Reactome: R-HSA-390471Reactome: R-HSA-8951664Reactome: R-HSA-9604323Reactome: R-HSA-983168 |
| Cellular Localisation | Isoform 1: NucleusNucleoplasmChromosomeLocalizes To Site Of Double-Strand Breaks Following Phosphorylation By AtmIsoform 2: CytoplasmIsoform 3: NucleusNucleolus |
| Alternative Antibody Names | Anti-F-Box/Wd Repeat-Containing Protein 7 antibodyAnti-Archipelago Homolog antibodyAnti-Hago antibodyAnti-F-Box And Wd-40 Domain-Containing Protein 7 antibodyAnti-F-Box Protein Fbx30 antibodyAnti-Sel-10 antibodyAnti-Hcdc4 antibodyAnti-FBXW7 antibodyAnti-FBW7 antibodyAnti-FBX30 antibodyAnti-SEL10 antibody |
Information sourced from Uniprot.org