• Western blot analysis of extracts of HeLa cells, using FBXO42 antibody (STJ117099) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-FBXO42 antibody (468-717) (STJ117099)

SKU:
STJ117099

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-FBXO42 (468-717) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: FBXO42
Gene ID: 54455
Uniprot ID: FBX42_HUMAN
Immunogen Region: 468-717
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 468-717 of human FBXO42 (NP_061867.1).
Immunogen Sequence: PSAPEGYDLKIGLSLAPRRG SLPDQKDLRLGSIDLNWDLK PASSSNPMDGMDNRTVGGSM RHPPEQTNGVHTPPHVASAL AGAVSPGALRRSLEAIKAMS SKGPSASAALSPPLGSSPGS PGSQSLSSGETVPIPRPGPA QGDGHSLPPIARRLGHHPPQ SLNVGKPLYQSMNCKPMQMY VLDIKDTKEKGRVKWKVFNS SSVVGPPETSLHTVVQGRGE LIIFGGLMDKKQNVKYYPK
Function Substrate-recognition component of some SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex. Specifically recognizes p53/TP53, promoting its ubiquitination and degradation.
Protein Name F-Box Only Protein 42
Just One F-Box And Kelch Domain-Containing Protein
Alternative Antibody Names Anti-F-Box Only Protein 42 antibody
Anti-Just One F-Box And Kelch Domain-Containing Protein antibody
Anti-FBXO42 antibody
Anti-FBX42 antibody
Anti-JFK antibody
Anti-KIAA1332 antibody

Information sourced from Uniprot.org

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