Anti-FBXO4 antibody (138-387) (STJ112009)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal FBXO4 (138-387) antibody for use in WB and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:1000-1:4000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | FBXO4 |
| Gene ID | 26272 |
| Uniprot ID | FBX4_HUMAN |
| Immunogen Region | 138-387 |
| Immunogen Sequence | DYMAVYRMCCPYTRRASKSS RPMYGAVTSFLHSLIIQNEP RFAMFGPGLEELNTSLVLSL MSSEELCPTAGLPQRQIDGI GSGVNFQLNNQHKFNILILY STTRKERDRAREEHTSAVNK MFSRHNEGDDQQGSRYSVIP QIQKVCEVVDGFIYVANAEA HKRHEWQDEFSHIMAMTDPA FGSSGRPLLVLSCISQGDVK RMPCFYLAHELHLNLLNHPW LVQDTEAETLTGFLNGIEW |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 138-387 of human FBXO4 (NP_036308.1). |
Additional Info
| Post Translational Modifications | Phosphorylation at Ser-12 varies during the cell cycle. It is low in resting cells and high in the S phase and the G2/M phase of the cell cycle. Phosphorylation is decreased during late G1 phase. Phosphorylation at Ser-12 promotes homodimerization and is necessary for optimal ubiquitin ligase activity towards CCND1. |
| Function | Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes ubiquitination of cyclin-D1 (CCND1) and its subsequent proteasomal degradation. However, it does not act as a major regulator of CCND1 stability during the G1/S transition. Recognizes TERF1 and promotes its ubiquitination together with UBE2D1. Promotes ubiquitination of FXR1 following phosphorylation of FXR1 by GSK3B, leading to FXR1 degradation by the proteasome. |
| Protein Name | F-Box Only Protein 4 |
| Database Links | Reactome: R-HSA-390471Reactome: R-HSA-8951664Reactome: R-HSA-983168 |
| Cellular Localisation | Cytoplasm |
| Alternative Antibody Names | Anti-F-Box Only Protein 4 antibodyAnti-FBXO4 antibodyAnti-FBX4 antibody |
Information sourced from Uniprot.org