Anti-FBXO3 antibody (1-240) (STJ118258)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal FBXO3 (1-240) antibody for use in WB and ELISA in mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | FBXO3 |
| Gene ID | 26273 |
| Uniprot ID | FBX3_HUMAN |
| Immunogen Region | 1-240 |
| Immunogen Sequence | MAAMETETAPLTLESLPTDP LLLILSFLDYRDLINCCYVS RRLSQLSSHDPLWRRHCKKY WLISEEEKTQKNQCWKSLFI DTYSDVGRYIDHYAAIKKAW DDLKKYLEPRCPRMVLSLKE GAREEDLDAVEAQIGCKLPD DYRCSYRIHNGQKLVVPGLL GSMALSNHYRSEDLLDVDTA AGGFQQRQGLKYCLPLTFCI HTGLSQYIAVEAAEGRNKNE VFYQCPDQMARNPAAIDMF |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human FBXO3 (NP_036307.2). |
Additional Info
| Function | Substrate recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex, SCF(FBXO3), which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Mediates the ubiquitination of HIPK2 and probably that of EP300, leading to rapid degradation by the proteasome. In the presence of PML, HIPK2 ubiquitination still occurs, but degradation is prevented. PML, HIPK2 and FBXO3 may act synergically to activate p53/TP53-dependent transactivation. The SCF(FBXO3) also acts as a regulator of inflammation by mediating ubiquitination and degradation of FBXL2 in response to lipopolysaccharide (LPS). The SCF(FBXO3) complex specifically recognizes FBXL2 phosphorylated at 'Thr-404' and promotes its ubiquitination. (Microbial infection) Associates with the Rift valley fever virus NSs to form a remodeled E3 ligase that triggers efficient proteasomal degradation of targeted proteins. The filamentous E3 ligase targets the TFIIH complex leading to robust inhibition of antiviral immunity and enhances viral pathogenesis. |
| Protein Name | F-Box Only Protein 3 |
| Database Links | |
| Cellular Localisation | NucleusColocalizes With Pml At The Peripheries Of Nuclear Bodies |
| Alternative Antibody Names | Anti-F-Box Only Protein 3 antibodyAnti-FBXO3 antibodyAnti-FBX3 antibody |
Information sourced from Uniprot.org