Anti-FBXO22 antibody (182-232 aa) (STJ195843)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from the human FBX22 at the amino acid range 182-232
General Information
| Short Description | Rabbit polyclonal anti-F-box only protein 22 (182-232 aa) for use in WB in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | FBXO22 |
| Gene ID | 26263 |
| Uniprot ID | FBX22_HUMAN |
| Immunogen | Synthesized peptide derived from the human FBX22 at the amino acid range 182-232 |
| Immunogen Region | 182-232 aa |
| Specificity | This antibody detects endogenous levels of FBX22 at Human/Mouse |
Additional Info
| Post Translational Modifications | Phosphorylated by EIF2AK4 at Thr-127 causes cytoplasmic retention of FBXO22. |
| Function | Substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex that is implicated in the control of various cellular processes such as cell cycle control, transcriptional regulation, DNA damage repair, and apoptosis. Promotes the proteasome-dependent degradation of key sarcomeric proteins, such as alpha-actinin (ACTN2) and filamin-C (FLNC), essential for maintenance of normal contractile function. Acts as a key regulator of histone methylation marks namely H3K9 and H3K36 methylation through the regulation of histone demethylase KDM4A protein levels. In complex with KDM4A, also regulates the abundance of TP53 by targeting methylated TP53 for degradation at the late senescent stage. Under oxidative stress, promotes the ubiquitination and degradation of BACH1. Mechanistically, reactive oxygen species (ROS) covalently modify cysteine residues on the bZIP domain of BACH1, leading to its release from chromatin and making it accessible to FBXO22. Upon amino acid depletion, mediates 'Lys-27'-linked ubiquitination of MTOR and thereby inhibits substrate recruitment to mTORC1. Also inhibits SARS-CoV-2 replication by inducing NSP5 degradation. |
| Protein Name | F-Box Only Protein 22F-Box Protein Fbx22p44 |
| Database Links | Reactome: R-HSA-8951664Reactome: R-HSA-983168 |
| Cellular Localisation | CytoplasmNucleusMyofibrilSarcomereZ LineAmino Acid Depletion Lead To A Time-Dependent Increase Of Fbxo22 In The Cytoplasm |
| Alternative Antibody Names | Anti-F-Box Only Protein 22 antibodyAnti-F-Box Protein Fbx22p44 antibodyAnti-FBXO22 antibodyAnti-FBX22 antibody |
Information sourced from Uniprot.org