| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-FBXL5 (1-310) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
FBXL5 |
| Gene ID: |
26234 |
| Uniprot ID: |
FBXL5_HUMAN |
| Immunogen Region: |
1-310 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-310 of human FBXL5 (NP_036293.1). |
| Immunogen Sequence: |
MAPFPEEVDVFTAPHWRMKQ LVGLYCDKLSKTNFSNNNDF RALLQSLYATFKEFKMHEQI ENEYIIGLLQQRSQTIYNVH SDNKLSEMLSLFEKGLKNVK NEYEQLNYAKQLKERLEAFT RDFLPHMKEEEEVFQPMLME YFTYEELKDIKKKVIAQHCS QKDTAELLRGLSLWNHAEER QKFFKYSVDEKSDKEAEVSE HSTGITHLPPEVMLSIFSYL NPQELCRCSQVSMKWSQLT |
| Post Translational Modifications | Polybiquitinated upon iron and oxygen depletion, leading to its degradation by the proteasome. Ubiquitination is regulated by the hemerythrin-like region that acts as an oxygen and iron sensor. Undergoes constitutive ubiquitin-dependent degradation at the steady state by HERC2. |
| Function | Component of some SCF (SKP1-cullin-F-box) protein ligase complex that plays a central role in iron homeostasis by promoting the ubiquitination and subsequent degradation of IREB2/IRP2. The C-terminal domain of FBXL5 contains a redox-sensitive 2Fe-2S cluster that, upon oxidation, promotes binding to IRP2 to effect its oxygen-dependent degradation. Under iron deficiency conditions, the N-terminal hemerythrin-like (Hr) region, which contains a diiron metal center, cannot bind iron and undergoes conformational changes that destabilize the FBXL5 protein and cause its ubiquitination and degradation. When intracellular iron levels start rising, the Hr region is stabilized. Additional increases in iron levels facilitate the assembly and incorporation of a redox active 2Fe-2S cluster in the C-terminal domain. Only when oxygen level is high enough to maintain the cluster in its oxidized state can FBXL5 recruit IRP2 as a substrate for polyubiquination and degradation. Promotes ubiquitination and subsequent degradation of the dynactin complex component DCTN1. Within the nucleus, promotes the ubiquitination of SNAI1.preventing its interaction with DNA and promoting its degradation. Negatively regulates DNA damage response by mediating the ubiquitin-proteasome degradation of the DNA repair protein NABP2. |
| Protein Name | F-Box/Lrr-Repeat Protein 5F-Box And Leucine-Rich Repeat Protein 5F-Box Protein Fbl4/Fbl5P45skp2-Like Protein |
| Database Links | Reactome: R-HSA-390471Reactome: R-HSA-8951664Reactome: R-HSA-917937Reactome: R-HSA-983168 |
| Cellular Localisation | CytoplasmPerinuclear RegionNucleus |
| Alternative Antibody Names | Anti-F-Box/Lrr-Repeat Protein 5 antibodyAnti-F-Box And Leucine-Rich Repeat Protein 5 antibodyAnti-F-Box Protein Fbl4/Fbl5 antibodyAnti-P45skp2-Like Protein antibodyAnti-FBXL5 antibodyAnti-FBL4 antibodyAnti-FBL5 antibodyAnti-FLR1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
