| Host: |
Rabbit |
| Applications: |
WB/IF/IP |
| Reactivity: |
Human/Mouse/Rat/Monkey |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-FBL (100-321) is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IF/ICC 1:50-1:200IP 1:20-1:50 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
FBL |
| Gene ID: |
2091 |
| Uniprot ID: |
FBRL_HUMAN |
| Immunogen Region: |
100-321 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 100-321 of human Fibrillarin/U3 RNP (NP_001427.2). |
| Immunogen Sequence: |
RGKEDALVTKNLVPGESVYG EKRVSISEGDDKIEYRAWNP FRSKLAAAILGGVDQIHIKP GAKVLYLGAASGTTVSHVSD IVGPDGLVYAVEFSHRSGRD LINLAKKRTNIIPVIEDARH PHKYRMLIAMVDVIFADVAQ PDQTRIVALNAHTFLRNGGH FVISIKANCIDSTASAEAVF ASEVKKMQQENMKPQEQLTL EPYERDHAVVVGVYRPPPKV KN |
| Post Translational Modifications | Ubiquitinated. Ubiquitination leads to proteasomal degradation. Deubiquitinated by USP36. By homology to other fibrillarins, some or all of the N-terminal domain arginines are modified to asymmetric dimethylarginine (DMA). Acetylated by CREBBP/CBP, preventing methylation of 'Gln-105' of histone H2A (H2AQ104me), without affecting rRNA methylation. Deacetylation by SIRT7 restores methylation of 'Gln-105' of histone H2A (H2AQ104me). |
| Function | S-adenosyl-L-methionine-dependent methyltransferase that has the ability to methylate both RNAs and proteins. Involved in pre-rRNA processing by catalyzing the site-specific 2'-hydroxyl methylation of ribose moieties in pre-ribosomal RNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA. Probably catalyzes 2'-O-methylation of U6 snRNAs in box C/D RNP complexes. U6 snRNA 2'-O-methylation is required for mRNA splicing fidelity. Also acts as a protein methyltransferase by mediating methylation of 'Gln-105' of histone H2A (H2AQ104me), a modification that impairs binding of the FACT complex and is specifically present at 35S ribosomal DNA locus. Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome. |
| Protein Name | Rrna 2'-O-Methyltransferase Fibrillarin34 Kda Nucleolar Scleroderma AntigenHistone-Glutamine MethyltransferaseU6 Snrna 2'-O-Methyltransferase Fibrillarin |
| Database Links | Reactome: R-HSA-6790901Reactome: R-HSA-6791226 |
| Cellular Localisation | NucleusNucleolusNucleoplasmFibrillar Region Of The Nucleolus |
| Alternative Antibody Names | Anti-Rrna 2'-O-Methyltransferase Fibrillarin antibodyAnti-34 Kda Nucleolar Scleroderma Antigen antibodyAnti-Histone-Glutamine Methyltransferase antibodyAnti-U6 Snrna 2'-O-Methyltransferase Fibrillarin antibodyAnti-FBL antibodyAnti-FIB1 antibodyAnti-FLRN antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
