| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-FANCI (1061-1328) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
FANCI |
| Gene ID: |
55215 |
| Uniprot ID: |
FANCI_HUMAN |
| Immunogen Region: |
1061-1328 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1061-1328 of human FANCI (NP_001106849.1). |
| Immunogen Sequence: |
DQDVEVEKTNHFAIVNLRTA APTVCLLVLSQAEKVLEEVD WLITKLKGQVSQETLSEEAS SQATLPNQPVEKAIIMQLGT LLTFFHELVQTALPSGSCVD TLLKDLCKMYTTLTALVRYY LQVCQSSGGIPKNMEKLVKL SGSHLTPLCYSFISYVQNKS KSLNYTGEKKEKPAAVATAM ARVLRETKPIPNLIFAIEQY EKFLIHLSKKSKVNLMQHMK LSTSRDFKIKGNILDMVLR |
| Post Translational Modifications | Monoubiquitinated by FANCL on Lys-523 during S phase and upon genotoxic stress. Deubiquitinated by USP1 as cells enter G2/M, or once DNA repair is completed. Monoubiquitination requires the FANCA-FANCB-FANCC-FANCE-FANCF-FANCG-FANCM complex. Ubiquitination is required for binding to chromatin, DNA repair, and normal cell cycle progression. Monoubiquitination is stimulated by DNA-binding. Phosphorylated in response to DNA damage by ATM and/or ATR. |
| Function | Plays an essential role in the repair of DNA double-strand breaks by homologous recombination and in the repair of interstrand DNA cross-links (ICLs) by promoting FANCD2 monoubiquitination by FANCL and participating in recruitment to DNA repair sites. Required for maintenance of chromosomal stability. Specifically binds branched DNA: binds both single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA). Participates in S phase and G2 phase checkpoint activation upon DNA damage. |
| Protein Name | Fanconi Anemia Group I ProteinProtein Faci |
| Database Links | Reactome: R-HSA-6783310Reactome: R-HSA-6796648 |
| Cellular Localisation | NucleusCytoplasmObserved In Spots Localized In Pairs On The Sister Chromatids Of Mitotic Chromosome Arms And Not CentromeresOne On Each ChromatidsThese Foci Coincide With Common Fragile SitesThey Are Frequently Interlinked Through Blm-Associated Ultra-Fine Dna Bridges |
| Alternative Antibody Names | Anti-Fanconi Anemia Group I Protein antibodyAnti-Protein Faci antibodyAnti-FANCI antibodyAnti-KIAA1794 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
