• Western blot analysis of extracts of various cell lines, using FAM20A antibody (STJ110794) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
  • Immunohistochemistry analysis of paraffin-embedded mouse lung using FAM20A antibody (STJ110794) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded rat lung using FAM20A antibody (STJ110794) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of paraffin-embedded human esophagus using FAM20A antibody (STJ110794) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human esophagus using FAM20A antibody (STJ110794) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of paraffin-embedded rat lung using FAM20A antibody (STJ110794) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse lung using FAM20A antibody (STJ110794) at dilution of 1:100 (40x lens).
  • Western blot analysis of extracts of various cell lines, using FAM20A antibody (STJ110794) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-FAM20A antibody (140-240) (STJ110794)

SKU:
STJ110794

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-FAM20A (140-240) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:100-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: FAM20A
Gene ID: 54757
Uniprot ID: FA20A_HUMAN
Immunogen Region: 140-240
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 140-240 of human FAM20A (NP_060035.2).
Immunogen Sequence: YREQMNLTSLDPPLQLRLEA SWVQFHLGINRHGLYSRSSP VVSKLLQDMRHFPTISADYS QDEKALLGACDCTQIVKPSG VHLKLVLRFSDFGKAMFKPM R
Tissue Specificity Highly expressed in lung and liver. Intermediate levels in thymus and ovary.
Post Translational Modifications N-glycosylated.
Function Pseudokinase that acts as an allosteric activator of the Golgi serine/threonine protein kinase FAM20C and is involved in biomineralization of teeth. Forms a complex with FAM20C and increases the ability of FAM20C to phosphorylate the proteins that form the 'matrix' that guides the deposition of the enamel minerals.
Protein Name Pseudokinase Fam20a
Database Links Reactome: R-HSA-381426
Reactome: R-HSA-8957275
Cellular Localisation Secreted
Golgi Apparatus
Endoplasmic Reticulum
Alternative Antibody Names Anti-Pseudokinase Fam20a antibody
Anti-FAM20A antibody
Anti-UNQ9388 antibody
Anti-PRO34279 antibody

Information sourced from Uniprot.org

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