• Western blot analysis of extracts of various cell lines, using FAM173B antibody (STJ29540) at 1:1000 dilution. _Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. _Lysates/proteins: 25 Mu g per lane. _Blocking buffer: 3% nonfat dry milk in TBST. _Detection: ECL Enhanced Kit. _Exposure time: 90s.

Anti-FAM173B antibody (1-233) (STJ29540)

SKU:
STJ29540

£169.50 - £449.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-FAM173B (1-233) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ATPSCKMT
Gene ID: 134145
Uniprot ID: ACKMT_HUMAN
Immunogen Region: 1-233
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-233 of human FAM173B (NP_954584.2).
Immunogen Sequence: MEGGGGIPLETLKEESQSRH VLPASFEVNSLQKSNWGFLL TGLVGGTLVAVYAVATPFVT PALRKVCLPFVPATTKQIEN VVKMLRCRRGSLVDIGSGDG RIVIAAAKKGFTAVGYELNP WLVWYSRYRAWREGVHGSAK FYISDLWKVTFSQYSNVVIF GVPQMMLQLEKKLERELEDD ARVIACRFPFPHWTPDHVTG EGIDTVWAYDASTFRGREKR PCTSMHFQLPIQA
Tissue Specificity Ubiquitously expressed.
Function Mitochondrial protein-lysine N-methyltransferase that trimethylates ATP synthase subunit C, ATP5MC1 and ATP5MC2. Trimethylation is required for proper incorporation of the C subunit into the ATP synthase complex and mitochondrial respiration. Promotes chronic pain. Involved in persistent inflammatory and neuropathic pain: methyltransferase activity in the mitochondria of sensory neurons promotes chronic pain via a pathway that depends on the production of reactive oxygen species (ROS) and on the engagement of spinal cord microglia.
Protein Name Atp Synthase Subunit C Lysine N-Methyltransferase
Protein N-Lysine Methyltransferase Fam173b
Hfam173b
Cellular Localisation Mitochondrion Membrane
Single-Pass Membrane Protein
Localizes To Mitochondrial Cristae
Alternative Antibody Names Anti-Atp Synthase Subunit C Lysine N-Methyltransferase antibody
Anti-Protein N-Lysine Methyltransferase Fam173b antibody
Anti-Hfam173b antibody
Anti-ATPSCKMT antibody
Anti-FAM173B antibody

Information sourced from Uniprot.org

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