• Immunoprecipitation analysis of 600 Mu g extracts of Mouse heart cells using 3 Mu g FADD antibody (STJ11103241). Western blot was performed from the immunoprecipitate using FADD antibody (STJ11103241) at a dilution of 1:1000.
  • Immunofluorescence analysis of paraffin-embedded mouse bone marrow using FADD Rabbit polyclonal antibody (STJ11103241) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of lysates from Jurkat cells, using FADD Rabbit polyclonal antibody (STJ11103241) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Western blot analysis of lysates from Mouse heart, using FADD Rabbit polyclonal antibody (STJ11103241) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-FADD antibody (1-205) (STJ11103241)

SKU:
STJ11103241

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-FADD (1-205) is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
IP 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: Fadd
Gene ID: 14082
Uniprot ID: FADD_MOUSE
Immunogen Region: 1-205
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-205 of mouse FADD (NP_034305.1).
Immunogen Sequence: MDPFLVLLHSLSGSLSGNDL MELKFLCRERVSKRKLERVQ SGLDLFTVLLEQNDLERGHT GLLRELLASLRRHDLLQRLD DFEAGTATAAPPGEADLQVA FDIVCDNVGRDWKRLARELK VSEAKMDGIEEKYPRSLSER VRESLKVWKNAEKKNASVAG LVKALRTCRLNLVADLVEEA QESVSKSENMSPVLRDSTVS SSETP
Function Apoptotic adaptor molecule that recruits caspase-8 or caspase-10 to the activated Fas (CD95) or TNFR-1 receptors. The resulting aggregate called the death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation. Active caspase-8 initiates the subsequent cascade of caspases mediating apoptosis. Involved in interferon-mediated antiviral immune response, playing a role in the positive regulation of interferon signaling.
Protein Name Fas-Associated Death Domain Protein
Fas-Associating Death Domain-Containing Protein
Mediator Of Receptor Induced Toxicity
Database Links Reactome: R-MMU-140534
Reactome: -MMU-2562578
Reactome: -MMU-3371378
Reactome: -MMU-5218900
Reactome: -MMU-5357786
Reactome: -MMU-5357905
Reactome: -MMU-5675482
Reactome: -MMU-69416
Reactome: -MMU-75157
Reactome: -MMU-75158
Alternative Antibody Names Anti-Fas-Associated Death Domain Protein antibody
Anti-Fas-Associating Death Domain-Containing Protein antibody
Anti-Mediator Of Receptor Induced Toxicity antibody
Anti-Fadd antibody
Anti-Mort1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance