Anti-FACT/SUPT16H antibody (Internal) (STJ70805)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-FACT/SUPT16H (Internal) is suitable for use in ELISA and Western Blot research applications. |
| Applications | Pep-ELISA/WB |
| Host | Goat |
| Reactivity | Human/Mouse/Pig/Cow |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx 150kDa band observed in lysates of cell line HeLa (calculated MW of 120kDa according to NP_009123.1). Recommended concentration: 0.1-0.3µg/ml. An additional band of unknown ident |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | SUPT16H |
| Gene ID | 11198 |
| Uniprot ID | SP16H_HUMAN |
| Accession Number | NP_009123.1 |
| Immunogen Region | Internal |
| Immunogen Sequence | DLTNKEGKKPEEK |
Additional Info
| Post Translational Modifications | ADP-ribosylated. ADP-ribosylation by PARP1 is induced by genotoxic stress and correlates with dissociation of FACT from chromatin. |
| Function | Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II. The FACT complex is probably also involved in phosphorylation of 'Ser-392' of p53/TP53 via its association with CK2 (casein kinase II). |
| Protein Name | Fact Complex Subunit Spt16Chromatin-Specific Transcription Elongation Factor 140 Kda SubunitFact 140 Kda SubunitFactp140Facilitates Chromatin Transcription Complex Subunit Spt16Hspt16 |
| Database Links | Reactome: R-HSA-112382Reactome: R-HSA-167152Reactome: R-HSA-167200Reactome: R-HSA-167238Reactome: R-HSA-167243Reactome: R-HSA-167246Reactome: R-HSA-167287Reactome: R-HSA-167290Reactome: R-HSA-674695Reactome: R-HSA-6796648Reactome: R-HSA-6804756Reactome: R-HSA-75955 |
| Cellular Localisation | NucleusChromosomeColocalizes With Rna Polymerase Ii On ChromatinRecruited To Actively Transcribed Loci |
| Alternative Antibody Names | Anti-Fact Complex Subunit Spt16 antibodyAnti-Chromatin-Specific Transcription Elongation Factor 140 Kda Subunit antibodyAnti-Fact 140 Kda Subunit antibodyAnti-Factp140 antibodyAnti-Facilitates Chromatin Transcription Complex Subunit Spt16 antibodyAnti-Hspt16 antibodyAnti-SUPT16H antibodyAnti-FACT140 antibodyAnti-FACTP140 antibody |
Information sourced from Uniprot.org