• Western blot analysis of extracts of HepG2 cells, using F5 antibody (STJ118027) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 240s.

Anti-F5 antibody (300-510) (STJ118027)

SKU:
STJ118027

£169.50 - £449.50
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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-F5 (300-510) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: F5
Gene ID: 2153
Uniprot ID: FA5_HUMAN
Immunogen Region: 300-510
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 300-510 of human F5 (NP_000121.2).
Immunogen Sequence: VGPEGKWIISSLTPKHLQAG MQAYIDIKNCPKKTRNLKKI TREQRRHMKRWEYFIAAEEV IWDYAPVIPANMDKKYRSQH LDNFSNQIGKHYKKVMYTQY EDESFTKHTVNPNMKEDGIL GPIIRAQVRDTLKIVFKNMA SRPYSIYPHGVTFSPYEDEV NSSFTSGRNNTMIRAVQPGE TYTYKWNILEFDEPTENDAQ CLTRPYYSDVD
Tissue Specificity Plasma.
Post Translational Modifications Thrombin activates factor V proteolytically to the active cofactor, factor Va (formation of a heavy chain at the N-terminus and a light chain at the C-terminus). Sulfation is required for efficient thrombin cleavage and activation and for full procoagulant activity. Activated protein C inactivates factor V and factor Va by proteolytic degradation. Phosphorylated by FAM20C in the extracellular medium.
Function Central regulator of hemostasis. It serves as a critical cofactor for the prothrombinase activity of factor Xa that results in the activation of prothrombin to thrombin.
Protein Name Coagulation Factor V
Activated Protein C Cofactor
Proaccelerin - Labile Factor Cleaved Into - Coagulation Factor V Heavy Chain - Coagulation Factor V Light Chain
Database Links Reactome: R-HSA-114608
Reactome: R-HSA-140875
Reactome: R-HSA-204005
Reactome: R-HSA-381426
Reactome: R-HSA-5694530
Reactome: R-HSA-8957275
Cellular Localisation Secreted
Alternative Antibody Names Anti-Coagulation Factor V antibody
Anti-Activated Protein C Cofactor antibody
Anti-Proaccelerin - Labile Factor Cleaved Into - Coagulation Factor V Heavy Chain - Coagulation Factor V Light Chain antibody
Anti-F5 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance