Western blot analysis of extracts of BxPC-3 cells, using Coagulation factor III/Tissue Factor antibody (STJ23598) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-F3 antibody (120-251) (STJ23598)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ23598
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General Information

Short DescriptionRabbit polyclonal F3 (120-251) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/IF/ICC/ELISA
HostRabbit
ReactivityHuman/Mouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:500-1:1000
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolF3
Gene ID2152
Uniprot IDTF_HUMAN
Immunogen Region120-251
Immunogen SequenceSAGEPLYENSPEFTPYLETN LGQPTIQSFEQVGTKVNVTV EDERTLVRRNNTFLSLRDVF GKDLIYTLYYWKSSSSGKKT AKTNTNEFLIDVDKGENYCF SVQAVIPSRTVNRKSTDSPV ECMGQEKGEFRE
SpecificityRecombinant fusion protein containing a sequence corresponding to amino acids 120-251 of human Coagulation factor III/Tissue Factor (NP_001984.1).

Additional Info

Tissue Specificity Lung, placenta and pancreas.
Function Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The TF:VIIa complex activates factors IX or X by specific limited proteolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade.
Protein Name Tissue Factor
Tf
Coagulation Factor Iii
Thromboplastin
Cd Antigen Cd142
Database Links Reactome: R-HSA-140834
Reactome: R-HSA-9031628
Cellular Localisation Isoform 1: Membrane
Single-Pass Type I Membrane Protein
Isoform 2: Secreted
Alternative Antibody Names Anti-Tissue Factor antibody
Anti-Tf antibody
Anti-Coagulation Factor Iii antibody
Anti-Thromboplastin antibody
Anti-Cd Antigen Cd142 antibody
Anti-F3 antibody

Information sourced from Uniprot.org

Citations

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