Anti-F12 antibody (336-615) (STJ23595)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal F12 (336-615) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | F12 |
| Gene ID | 2161 |
| Uniprot ID | FA12_HUMAN |
| Immunogen Region | 336-615 |
| Immunogen Sequence | QTPGALPAKREQPPSLTRNG PLSCGQRLRKSLSSMTRVVG GLVALRGAHPYIAALYWGHS FCAGSLIAPCWVLTAAHCLQ DRPAPEDLTVVLGQERRNHS CEPCQTLAVRSYRLHEAFSP VSYQHDLALLRLQEDADGSC ALLSPYVQPVCLPSGAARPS ETTLCQVAGWGHQFEGAEEY ASFLQEAQVPFLSLERCSAP DVHGSSILPGMLCAGFLEGG TDACQGDSGGPLVCEDQAA |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 336-615 of human F12 (NP_000496.2). |
Additional Info
| Post Translational Modifications | Factor XII is activated by kallikrein in alpha-factor XIIa, which is further converted by trypsin into beta-factor XIIa. Alpha-factor XIIa is composed of an NH2-terminal heavy chain, called coagulation factor XIIa heavy chain, and a COOH-terminal light chain, called coagulation factor XIIa light chain, connected by a disulfide bond. Beta-factor XIIa is composed of 2 chains linked by a disulfide bond, an N-terminal nonapeptide, called beta-factor XIIa part 1, and coagulation factor XIIa light chain, also known in this context as beta-factor XIIa part 2. O- and N-glycosylated. The O-linked polysaccharides were not identified, but are probably the mucin type linked to GalNAc. |
| Function | Factor XII is a serum glycoprotein that participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII first to alpha-factor XIIa and then trypsin cleaves it to beta-factor XIIa. Alpha-factor XIIa activates factor XI to factor XIa. |
| Protein Name | Coagulation Factor XiiHageman FactorHaf Cleaved Into - Coagulation Factor Xiia Heavy Chain - Beta-Factor Xiia Part 1 - Coagulation Factor Xiia Light ChainBeta-Factor Xiia Part 2 |
| Database Links | Reactome: R-HSA-140837Reactome: R-HSA-9657688Reactome: R-HSA-9657689 |
| Cellular Localisation | Secreted |
| Alternative Antibody Names | Anti-Coagulation Factor Xii antibodyAnti-Hageman Factor antibodyAnti-Haf Cleaved Into - Coagulation Factor Xiia Heavy Chain - Beta-Factor Xiia Part 1 - Coagulation Factor Xiia Light Chain antibodyAnti-Beta-Factor Xiia Part 2 antibodyAnti-F12 antibody |
Information sourced from Uniprot.org