• Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using Ezrin Rabbit monoclonal antibody (STJ11101586) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of HCT116 cells, using Ezrin antibody (STJ11101586) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-EZR antibody (300-400) [S6MR] (STJ11101586)

SKU:
STJ11101586

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Ezrin (300-400) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S6MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EZR
Gene ID: 7430
Uniprot ID: EZRI_HUMAN
Immunogen Region: 300-400
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 300-400 of human Ezrin (P15311).
Immunogen Sequence: IEVQQMKAQAREEKHQKQLE RQQLETEKKRRETVEREKEQ MMREKEELMLRLQDYEEKTK KAERELSEQIQRALQLEEER KRAQEEAERLEADRMAALRA K
Tissue Specificity Expressed in cerebral cortex, basal ganglia, hippocampus, hypophysis, and optic nerve. Weakly expressed in brain stem and diencephalon. Stronger expression was detected in gray matter of frontal lobe compared to white matter (at protein level). Component of the microvilli of intestinal epithelial cells. Preferentially expressed in astrocytes of hippocampus, frontal cortex, thalamus, parahippocampal cortex, amygdala, insula, and corpus callosum. Not detected in neurons in most tissues studied.
Post Translational Modifications Phosphorylated by tyrosine-protein kinases. Phosphorylation by ROCK2 suppresses the head-to-tail association of the N-terminal and C-terminal halves resulting in an opened conformation which is capable of actin and membrane-binding. S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
Function Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis.
Protein Name Ezrin
Cytovillin
Villin-2
P81
Database Links Reactome: R-HSA-373752
Reactome: R-HSA-437239
Reactome: R-HSA-9662360
Reactome: R-HSA-9662361
Cellular Localisation Apical Cell Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Cell Projection
Microvillus Membrane
Ruffle Membrane
Cytoplasm
Cell Cortex
Cytoskeleton
Microvillus
Localization To The Apical Membrane Of Parietal Cells Depends On The Interaction With Pals1
Localizes To Cell Extensions And Peripheral Processes Of Astrocytes
Microvillar Peripheral Membrane Protein (Cytoplasmic Side)
Alternative Antibody Names Anti-Ezrin antibody
Anti-Cytovillin antibody
Anti-Villin-2 antibody
Anti-P81 antibody
Anti-EZR antibody
Anti-VIL2 antibody

Information sourced from Uniprot.org

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