Anti-EYA1/EYA4 antibody (271-320 aa) (STJ99013)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthetic peptide from the human protein at the amino acid range 271-320
General Information
| Short Description | Rabbit polyclonal anti-Eyes absent homolog 1 and Eyes absent homolog 4 (271-320 aa) for use in IHC, IF and ELISA in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | IHC-P 1:50-200ELISA 1:10000-20000IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | EYA1EYA4 |
| Gene ID | 21382070 |
| Uniprot ID | EYA1_HUMANEYA4_HUMAN |
| Immunogen | Synthetic peptide from the human protein at the amino acid range 271-320 |
| Immunogen Region | 271-320 aa |
| Specificity | The antibody detects endogenous EYA1/EYA4 |
Additional Info
| Post Translational Modifications | Sumoylated with SUMO1. |
| Function | Functions both as protein phosphatase and as transcriptional coactivator for SIX1, and probably also for SIX2, SIX4 and SIX5. Tyrosine phosphatase that dephosphorylates 'Tyr-142' of histone H2AX (H2AXY142ph) and promotes efficient DNA repair via the recruitment of DNA repair complexes containing MDC1. 'Tyr-142' phosphorylation of histone H2AX plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress. Its function as histone phosphatase may contribute to its function in transcription regulation during organogenesis. Has also phosphatase activity with proteins phosphorylated on Ser and Thr residues (in vitro). Required for normal embryonic development of the craniofacial and trunk skeleton, kidneys and ears. Together with SIX1, it plays an important role in hypaxial muscle development.in this it is functionally redundant with EYA2. |
| Protein Name | Eyes Absent Homolog 1 |
| Database Links | Reactome: R-HSA-5693565 |
| Cellular Localisation | CytoplasmNucleusLocalizes At Sites Of Dna Damage At Double-Strand Breaks (Dsbs) |
| Alternative Antibody Names | Anti-Eyes Absent Homolog 1 antibodyAnti-EYA1 antibody |
Information sourced from Uniprot.org