• Western blot analysis of extracts of various cell lines, using EXT1 antibody (STJ23589) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST.
  • Immunofluorescence analysis of HeLa cells using EXT1 rabbit polyclonal antibody (STJ23589) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using EXT1 rabbit polyclonal antibody (STJ23589) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of PC-12 cells using EXT1 rabbit polyclonal antibody (STJ23589) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U2OS cells using EXT1 rabbit polyclonal antibody (STJ23589) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-EXT1 antibody (467-746) (STJ23589)

SKU:
STJ23589

€140.63 - €653.13
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-EXT1 (467-746) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EXT1
Gene ID: 2131
Uniprot ID: EXT1_HUMAN
Immunogen Region: 467-746
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 467-746 of human EXT1 (NP_000118.2).
Immunogen Sequence: YYYANLGLKPPSKFTAVIHA VTPLVSQSQPVLKLLVAAAK SQYCAQIIVLWNCDKPLPAK HRWPATAVPVVVIEGESKVM SSRFLPYDNIITDAVLSLDE DTVLSTTEVDFAFTVWQSFP ERIVGYPARSHFWDNSKERW GYTSKWTNDYSMVLTGAAIY HKYYHYLYSHYLPASLKNMV DQLANCEDILMNFLVSAVTK LPPIKVTQKKQYKETMMGQT SRASRWADPDHFAQRQSCM
Tissue Specificity Ubiquitous.
Function Glycosyltransferase required for the biosynthesis of heparan-sulfate. The EXT1/EXT2 complex possesses substantially higher glycosyltransferase activity than EXT1 or EXT2 alone. Appears to be a tumor suppressor. Required for the exosomal release of SDCBP, CD63 and syndecan.
Protein Name Exostosin-1
Glucuronosyl-N-Acetylglucosaminyl-Proteoglycan/N-Acetylglucosaminyl-Proteoglycan 4-Alpha-N-Acetylglucosaminyltransferase
Multiple Exostoses Protein 1
Putative Tumor Suppressor Protein Ext1
Database Links Reactome: R-HSA-2022928
Reactome: R-HSA-3656237
Reactome: R-HSA-3656253
Cellular Localisation Endoplasmic Reticulum Membrane
Single-Pass Type Ii Membrane Protein
Golgi Apparatus Membrane
Golgi Apparatus
Cis-Golgi Network Membrane
The Ext1/Ext2 Complex Is Localized In The Golgi Apparatus
Alternative Antibody Names Anti-Exostosin-1 antibody
Anti-Glucuronosyl-N-Acetylglucosaminyl-Proteoglycan/N-Acetylglucosaminyl-Proteoglycan 4-Alpha-N-Acetylglucosaminyltransferase antibody
Anti-Multiple Exostoses Protein 1 antibody
Anti-Putative Tumor Suppressor Protein Ext1 antibody
Anti-EXT1 antibody

Information sourced from Uniprot.org

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