• Immunoprecipitation analysis of 300 Mu g extracts of HeLa cells using 3 Mu g EXOC2 antibody (STJ11104417). Western blot was performed from the immunoprecipitate using EXOC2 antibody (STJ11104417) at a dilution of 1:1000.
  • Immunofluorescence analysis of U2OS cells using EXOC2 Rabbit polyclonal antibody (STJ11104417) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using EXOC2 Rabbit polyclonal antibody (STJ11104417) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-EXOC2 antibody (1-220) (STJ11104417)

SKU:
STJ11104417

£116.50 - £543.50
Current Stock:
Host: Rabbit
Applications: WB/IF/IP
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Exocyst Complex Component 2 (1-220) is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
IP 0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cells
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EXOC2
Gene ID: 55770
Uniprot ID: EXOC2_HUMAN
Immunogen Region: 1-220
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human EXOC2 (NP_060773.3).
Immunogen Sequence: MSRSRQPPLVTGISPNEGIP WTKVTIRGENLGTGPTDLIG LTICGHNCLLTAEWMSASKI VCRVGQAKNDKGDIIVTTKS GGRGTSTVSFKLLKPEKIGI LDQSAVWVDEMNYYDMRTDR NKGIPPLSLRPANPLGIEIE KSKFSQKDLEMLFHGMSADF TSENFSAAWYLIENHSNTSF EQLKMAVTNLKRQANKKSEG SLAYVKGGLSTFFEAQDALS
Tissue Specificity Widely expressed with highest levels in brain and placenta.
Function Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane.
Protein Name Exocyst Complex Component 2
Exocyst Complex Component Sec5
Database Links Reactome: R-HSA-1445148
Reactome: R-HSA-264876
Reactome: R-HSA-5620916
Cellular Localisation Midbody
Midbody Ring
Recruitment To The Midbody Does Not Require Rala
Nor Ralb
Colocalizes With Cntrl/Centriolin At The Midbody Ring
Alternative Antibody Names Anti-Exocyst Complex Component 2 antibody
Anti-Exocyst Complex Component Sec5 antibody
Anti-EXOC2 antibody
Anti-SEC5 antibody
Anti-SEC5L1 antibody

Information sourced from Uniprot.org

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