• Biotinylated STJ71832 (0. 1ug/ml) staining of HeLa lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.

Anti-EWS/EWSR1, Biotinylated antibody (Internal) {Biotin} (STJ73491)

SKU:
STJ73491-100

€379.38
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Host: Goat
Applications: Pep-ELISA/WB
Reactivity: Human/Mouse/Rat/Pig/Cow
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-EWS/EWSR1, Biotinylated (Internal) is suitable for use in ELISA and Western Blot research applications.
Clonality: Polyclonal
Conjugation: Biotin
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-0.3-1µg/ml
ELISA-antibody detection limit dilution 1:64000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: EWSR1
Gene ID: 2130
Uniprot ID: EWS_HUMAN
Immunogen Region: Internal
Accession Number: NP_053733.2; NP_005234.1; NP_001156757.1; NP_001156758.1; NP_001156759.1
Specificity: This antibody is expected to recognize all five reported isoforms (NP_053733.2; NP_005234.1; NP_001156757.1; NP_001156758.1; NP_001156759.1).
Immunogen Sequence: TSYDQSSYSQQNTYG.
Post Translational Modifications Phosphorylated.calmodulin-binding inhibits phosphorylation of Ser-266. Highly methylated on arginine residues. Methylation is mediated by PRMT1 and, at lower level by PRMT8.
Function Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes.
Protein Name Rna-Binding Protein Ews
Ews Oncogene
Ewing Sarcoma Breakpoint Region 1 Protein
Cellular Localisation Nucleus
Cytoplasm
Cell Membrane
Relocates From Cytoplasm To Ribosomes Upon Ptk2b/Fak2 Activation
Alternative Antibody Names Anti-Rna-Binding Protein Ews antibody
Anti-Ews Oncogene antibody
Anti-Ewing Sarcoma Breakpoint Region 1 Protein antibody
Anti-EWSR1 antibody
Anti-EWS antibody

Information sourced from Uniprot.org

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