• Immunohistochemistry analysis of EVA1A in paraffin-embedded rat kidney using EVA1A Rabbit polyclonal antibody (STJ11105736) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from 293T cells, using EVA1A Rabbit polyclonal antibody (STJ11105736) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-EVA1A antibody (90-152) (STJ11105736)

SKU:
STJ11105736

Current Stock:
Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Protein Eva-1 Homolog A (90-152) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EVA1A
Gene ID: 84141
Uniprot ID: EVA1A_HUMAN
Immunogen Region: 90-152
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 90-152 of human EVA1A (NP_115557.1).
Immunogen Sequence: DTVSDLSVRRHRRFERTLNK NVFTSAEELERAQRLEERER IIREIWMNGQPEVPGTRSLN RYY
Tissue Specificity Expressed in lung, kidney, liver, pancreas, placenta, but not in heart and skeletal muscle.
Function Acts as a regulator of programmed cell death, mediating both autophagy and apoptosis.
Protein Name Protein Eva-1 Homolog A
Protein Fam176a
Transmembrane Protein 166
Database Links Reactome: R-HSA-381426
Reactome: R-HSA-8957275
Cellular Localisation Endoplasmic Reticulum Membrane
Single-Pass Membrane Protein
Lysosome Membrane
Alternative Antibody Names Anti-Protein Eva-1 Homolog A antibody
Anti-Protein Fam176a antibody
Anti-Transmembrane Protein 166 antibody
Anti-EVA1A antibody
Anti-FAM176A antibody
Anti-TMEM166 antibody
Anti-SP24 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance