![Western blot analysis of lysates from SH-SY5Y cells using [KD Validated] ETV1 Rabbit mAb (STJ11107174) at 1:1000 dilution incubated overnight at 4C. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 45s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/186370/408075/STJ11107174_1__85519.1744894498.jpg?c=1 "Western blot analysis of lysates from SH-SY5Y cells using [KD Validated] ETV1 Rabbit mAb (STJ11107174) at 1:1000 dilution incubated overnight at 4C. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 45s.")
![Western blot analysis of lysates from wild type (WT) and ETV1 knockdown (KD) HeLa cells using [KD Validated] ETV1 Rabbit mAb (STJ11107174) at 1:1000 dilution incubated overnight at 4C. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 45s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/186370/408076/STJ11107174_2__15736.1744894499.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and ETV1 knockdown (KD) HeLa cells using [KD Validated] ETV1 Rabbit mAb (STJ11107174) at 1:1000 dilution incubated overnight at 4C. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 45s.")
![Immunoprecipitation of ETV1 from 600 µg extracts of Mouse brain was performed using 1 µg of [KD Validated] ETV1 Rabbit mAb (STJ11107174). Rabbit IgG isotype control (STJ11105763) was used to precipitate the Control IgG sample. IP samples were eluted with 1X reducing Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using ETV1 Rabbit mAb (STJ11107174) at a dilution of 1:5000.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/186370/408077/STJ11107174_3__47392.1744894499.jpg?c=1 "Immunoprecipitation of ETV1 from 600 µg extracts of Mouse brain was performed using 1 µg of [KD Validated] ETV1 Rabbit mAb (STJ11107174). Rabbit IgG isotype control (STJ11105763) was used to precipitate the Control IgG sample. IP samples were eluted with 1X reducing Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using ETV1 Rabbit mAb (STJ11107174) at a dilution of 1:5000.")
![Chromatin immunoprecipitation was performed with 10 Mu g of cross-linked chromatin from K-562, using 5 Mu g of [KD Validated] ETV1 Rabbit mAb (STJ11107174) and Rabbit IgG isotype control (STJ11105763). The enrichment of immunoprecipitated DNA at different genomic loci was examined by quantitative PCR. The histogram compares the ratio of the immunoprecipitated DNA to the input at given loci.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/186370/408078/STJ11107174_4__08758.1744894500.png?c=1 "Chromatin immunoprecipitation was performed with 10 Mu g of cross-linked chromatin from K-562, using 5 Mu g of [KD Validated] ETV1 Rabbit mAb (STJ11107174) and Rabbit IgG isotype control (STJ11105763). The enrichment of immunoprecipitated DNA at different genomic loci was examined by quantitative PCR. The histogram compares the ratio of the immunoprecipitated DNA to the input at given loci.")
![Western blot analysis of lysates from SH-SY5Y cells using [KD Validated] ETV1 Rabbit mAb (STJ11107174) at 1:1000 dilution incubated overnight at 4C. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 45s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/186370/408075/STJ11107174_1__85519.1744894498.jpg?c=1 "Western blot analysis of lysates from SH-SY5Y cells using [KD Validated] ETV1 Rabbit mAb (STJ11107174) at 1:1000 dilution incubated overnight at 4C. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 45s.")
Anti-ETV1 antibody (1-100 aa) [SRM5272] (STJ11107174)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal ETV1 (1-100 aa) antibody for use in WB, IP, ELISA and ChIP in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IP/ELISA/ChIP |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | SRM5272 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IP:0.5 Mu g-4 Mu g antibody for 400 Mu g-800 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.ChIP:5 Mu g anti |
| Formulation | PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ETV1 |
| Gene ID | 2115 |
| Uniprot ID | ETV1_HUMAN |
| Immunogen Region | 1-100 aa |
| Immunogen Sequence | MDGFYDQQVPYMVTNSQRGR NCNEKPTNVRKRKFINRDLA HDSEELFQDLSQLQETWLAE AQVPDNDEQFVPDYQAESLA FHGLPLKIKKEPHSPCSEIS |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human ETV1 (NP_004947.2). |
Additional Info
| Tissue Specificity | Very highly expressed in brain, highly expressed in testis, lung and heart, moderately in spleen, small intestine, pancreas and colon, weakly in liver, prostate and thymus, very weakly in skeletal muscle, kidney and ovary and not in placenta and peripheral blood leukocytes. |
| Post Translational Modifications | Sumoylated. Phosphorylated at Ser-191 and Ser-216 by RPS6KA1 and RPS6KA5.phosphorylation activates transcriptional activity. |
| Function | Transcriptional activator that binds to DNA sequences containing the consensus pentanucleotide 5'-CGGAAT-3'. Required for olfactory dopaminergic neuron differentiation.may directly activate expression of tyrosine hydroxylase (TH). |
| Protein Name | Ets Translocation Variant 1Ets-Related Protein 81 |
| Database Links | |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Ets Translocation Variant 1 antibodyAnti-Ets-Related Protein 81 antibodyAnti-ETV1 antibodyAnti-ER81 antibody |
Information sourced from Uniprot.org