• Immunofluorescence analysis of U-2 OS cells using ETS2 antibody (STJ29468) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using SPRY3 antibody (STJ29468) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-ETS2 antibody (76-469) (STJ29468)

SKU:
STJ29468

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ETS2 (76-469) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ETS2
Gene ID: 2114
Uniprot ID: ETS2_HUMAN
Immunogen Region: 76-469
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 76-469 of human ETS2 (NP_005230.1).
Immunogen Sequence: KAVMSQALKATFSGFKKEQR RLGIPKNPWLWSEQQVCQWL LWATNEFSLVNVNLQRFGMN GQMLCNLGKERFLELAPDFV GDILWEHLEQMIKEN
Post Translational Modifications Phosphorylation by CDK10 at Ser-220 and Ser-225 creates a phosphodegron that targets ETS2 for proteasomal degradation.
Function Transcription factor activating transcription. Binds specifically the DNA GGAA/T core motif (Ets-binding site or EBS) in gene promoters and stimulates transcription.
Protein Name Protein C-Ets-2
Database Links Reactome: R-HSA-2559585
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Protein C-Ets-2 antibody
Anti-ETS2 antibody

Information sourced from Uniprot.org

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