| Host: | Goat |
| Applications: | Pep-ELISA/WB |
| Reactivity: | Human/Mouse/Rat/Dog/Cow |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-ETFA (C-Term) is suitable for use in ELISA and Western Blot research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx 30kDa band observed in Human, Mouse and Rat Heart lysates (calculated MW of 30.0kDa according to NP_001121188.1). Recommended concentration: 0.05-0.2µg/ml. |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | ETFA |
| Gene ID: | 2108 |
| Uniprot ID: | ETFA_HUMAN |
| Immunogen Region: | C-Term |
| Accession Number: | NP_000117.1; NP_001121188.1 |
| Specificity: | This antibody is expected to recognize both reported isoforms (NP_000117.1; NP_001121188.1). |
| Immunogen Sequence: | QVADYGIVADLFK |
| Function | Heterodimeric electron transfer flavoprotein that accepts electrons from several mitochondrial dehydrogenases, including acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase). Required for normal mitochondrial fatty acid oxidation and normal amino acid metabolism. |
| Protein Name | Electron Transfer Flavoprotein Subunit Alpha - MitochondrialAlpha-Etf |
| Database Links | Reactome: R-HSA-611105 |
| Cellular Localisation | Mitochondrion Matrix |
| Alternative Antibody Names | Anti-Electron Transfer Flavoprotein Subunit Alpha - Mitochondrial antibodyAnti-Alpha-Etf antibodyAnti-ETFA antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and ETFA knockout (KO) 293T cells, using [KO Validated] ETFA Rabbit polyclonal antibody (STJ11100813) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/89749/358226/STJ11100813_1__44625.1713122552.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and ETFA knockout (KO) 293T cells, using [KO Validated] ETFA Rabbit polyclonal antibody (STJ11100813) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
