Anti-ETF1 antibody (STJA0009681)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from human ETF1
General Information
| Short Description | Rabbit polyclonal anti-Eukaryotic peptide chain release factor subunit 1 for use in WB in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ETF1 |
| Gene ID | 2107 |
| Uniprot ID | ERF1_HUMAN |
| Immunogen | Synthesized peptide derived from human ETF1 |
| Specificity | This antibody detects endogenous levels of ETF1 at Human, Mouse, Rat |
Additional Info
| Function | Component of the eRF1-eRF3-GTP ternary complex, a ternary complex that mediates translation termination in response to the termination codons. The eRF1-eRF3-GTP complex binds to a stop codon in the ribosomal A-site. ETF1/ERF1 is responsible for stop codon recognition and inducing hydrolysis of peptidyl-tRNA. Following GTP hydrolysis, eRF3 (GSPT1/ERF3A or GSPT2/ERF3B) dissociates, permitting ETF1/eRF1 to accommodate fully in the A-site and mediate hydrolysis of peptidyl-tRNA. Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. Required for SHFL-mediated translation termination which inhibits programmed ribosomal frameshifting (-1PRF) of mRNA from viruses and cellular genes. |
| Protein Name | Eukaryotic Peptide Chain Release Factor Subunit 1Eukaryotic Release Factor 1Erf1Protein Cl1Tb3-1 |
| Database Links | Reactome: R-HSA-72764Reactome: R-HSA-9010553Reactome: R-HSA-9629569Reactome: R-HSA-975956Reactome: R-HSA-975957 |
| Cellular Localisation | Cytoplasm |
| Alternative Antibody Names | Anti-Eukaryotic Peptide Chain Release Factor Subunit 1 antibodyAnti-Eukaryotic Release Factor 1 antibodyAnti-Erf1 antibodyAnti-Protein Cl1 antibodyAnti-Tb3-1 antibodyAnti-ETF1 antibodyAnti-ERF1 antibodyAnti-RF1 antibodyAnti-SUP45L1 antibody |
Information sourced from Uniprot.org