• Western blot analysis of extracts of various cell lines, using ERMAP antibody (STJ112457) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.

Anti-ERMAP antibody (30-155) (STJ112457)

SKU:
STJ112457

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ERMAP (30-155) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ERMAP
Gene ID: 114625
Uniprot ID: ERMAP_HUMAN
Immunogen Region: 30-155
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 30-155 of human ERMAP (NP_061008.2).
Immunogen Sequence: HAGDAGKFHVALLGGTAELL CPLSLWPGTVPKEVRWLRSP FPQRSQAVHIFRDGKDQDED LMPEYKGRTVLVRDAQEGSV TLQILDVRLEDQGSYRCLIQ VGNLSKEDTVILQVAAPSVG SLSPSA
Tissue Specificity Expressed in erythroid-enriched bone marrow (at protein level). Highly expressed in bone marrow and to a lower extent in leukocytes, thymus, lymph node and spleen.
Post Translational Modifications Glycosylated.
Function Possible role as a cell-adhesion or receptor molecule of erythroid cells.
Protein Name Erythroid Membrane-Associated Protein
Hermap
Radin Blood Group Antigen
Scianna Blood Group Antigen
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Cytoplasm
Alternative Antibody Names Anti-Erythroid Membrane-Associated Protein antibody
Anti-Hermap antibody
Anti-Radin Blood Group Antigen antibody
Anti-Scianna Blood Group Antigen antibody
Anti-ERMAP antibody
Anti-RD antibody
Anti-SC antibody

Information sourced from Uniprot.org

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