Anti-ERCC8 antibody [R02-7T5] (STJA0037444)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthesized peptide derived from human ERCC8
General Information
| Short Description | Rabbit monoclonal anti-ERCC8 for use in WB and IP in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IP |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | R02-7T5 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Affinity Purified |
| Dilution Range | WB 1:500-1:1000IP 1:50 |
| Formulation | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | ERCC8 |
| Gene ID | 1161 |
| Uniprot ID | ERCC8_HUMAN |
| Immunogen | A synthesized peptide derived from human ERCC8 |
Additional Info
| Function | Substrate-recognition component of the CSA complex, a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex, involved in transcription-coupled nucleotide excision repair (TC-NER), a process during which RNA polymerase II-blocking lesions are rapidly removed from the transcribed strand of active genes. Following recruitment to lesion-stalled RNA polymerase II (Pol II), the CSA complex mediates ubiquitination of Pol II subunit POLR2A/RPB1 at 'Lys-1268', a critical TC-NER checkpoint, governing RNA Pol II stability and initiating DNA damage excision by TFIIH recruitment. The CSA complex also promotes the ubiquitination and subsequent proteasomal degradation of ERCC6/CSB in a UV-dependent manner.ERCC6 degradation is essential for the recovery of RNA synthesis after transcription-coupled repair. Also plays a role in DNA double-strand breaks (DSSBs) repair by non-homologous end joining (NHEJ). |
| Protein Name | Dna Excision Repair Protein Ercc-8Cockayne Syndrome Wd Repeat Protein Csa |
| Database Links | Reactome: R-HSA-6781823Reactome: R-HSA-6781827Reactome: R-HSA-6782135Reactome: R-HSA-6782210Reactome: R-HSA-8951664 |
| Cellular Localisation | NucleusChromosomeNucleus MatrixRecruited To Lesion-Stalled Rna Polymerase Ii (Pol Ii) Sites By Ercc6/CsbUv-Induced Translocation To The Nuclear Matrix Is Dependent On Ercc6/Csb |
| Alternative Antibody Names | Anti-Dna Excision Repair Protein Ercc-8 antibodyAnti-Cockayne Syndrome Wd Repeat Protein Csa antibodyAnti-ERCC8 antibodyAnti-CKN1 antibodyAnti-CSA antibody |
Information sourced from Uniprot.org