Anti-ERCC5 antibody (1-270) (STJ118490)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal ERCC5 (1-270) antibody for use in IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ERCC5 |
| Gene ID | 2073 |
| Uniprot ID | ERCC5_HUMAN |
| Immunogen Region | 1-270 |
| Immunogen Sequence | MGVQGLWKLLECSGRQVSPE ALEGKILAVDISIWLNQALK GVRDRHGNSIENPHLLTLFH RLCKLLFFRIRPIFVFDGDA PLLKKQTLVKRRQRKDLASS DSRKTTEKLLKTFLKRQAIK TAFRSKRDEALPSLTQVRRE NDLYVLPPLQEEEKHSSEEE DEKEWQERMNQKQALQEEFF HNPQAIDIESEDFSSLPPEV KHEILTDMKEFTKRRRTLFE AMPEESDDFSQYQLKGLLK |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human ERCC5 (NP_000114.2). |
Additional Info
| Function | Single-stranded structure-specific DNA endonuclease involved in DNA excision repair. Makes the 3'incision in DNA nucleotide excision repair (NER). Binds and bends DNA repair bubble substrate and breaks base stacking at the single-strand/double-strand DNA junction of the DNA bubble. Plays a role in base excision repair (BER) by promoting the binding of DNA glycosylase NTHL1 to its substrate and increasing NTHL1 catalytic activity that removes oxidized pyrimidines from DNA. Involved in transcription-coupled nucleotide excision repair (TCR) which allows RNA polymerase II-blocking lesions to be rapidly removed from the transcribed strand of active genes. Functions during the initial step of TCR in cooperation with ERCC6/CSB to recognized stalled RNA polymerase II. Also, stimulates ERCC6/CSB binding to the DNA repair bubble and ERCC6/CSB ATPase activity. Required for DNA replication fork maintenance and preservation of genomic stability. Involved in homologous recombination repair (HRR) induced by DNA replication stress by recruiting RAD51, BRCA2, and PALB2 to the damaged DNA site. In TFIIH stimulates the 5'-3' helicase activity of XPD/ERCC2 and the DNA translocase activity of XPB/ERCC3. During HRR, binds to the replication fork with high specificity and stabilizes it. Also, acts upstream of HRR, to promote the release of BRCA1 from DNA. |
| Protein Name | Dna Excision Repair Protein Ercc-5Dna Repair Protein Complementing Xp-G CellsXpgXeroderma Pigmentosum Group G-Complementing Protein |
| Database Links | Reactome: R-HSA-5696395Reactome: R-HSA-5696400Reactome: R-HSA-6782135 |
| Cellular Localisation | NucleusChromosomeColocalizes With Rad51 To Nuclear Foci In S PhaseLocalizes To Dna Double-Strand Breaks (Dbs) During Replication StressColocalizes With Brca2 To Nuclear Foci Following Dna Replication Stress |
| Alternative Antibody Names | Anti-Dna Excision Repair Protein Ercc-5 antibodyAnti-Dna Repair Protein Complementing Xp-G Cells antibodyAnti-Xpg antibodyAnti-Xeroderma Pigmentosum Group G-Complementing Protein antibodyAnti-ERCC5 antibodyAnti-ERCM2 antibodyAnti-XPG antibodyAnti-XPGC antibody |
Information sourced from Uniprot.org