| Host: |
Rabbit |
| Applications: |
IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-ERCC5 (1-270) is suitable for use in Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
ERCC5 |
| Gene ID: |
2073 |
| Uniprot ID: |
ERCC5_HUMAN |
| Immunogen Region: |
1-270 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human ERCC5 (NP_000114.2). |
| Immunogen Sequence: |
MGVQGLWKLLECSGRQVSPE ALEGKILAVDISIWLNQALK GVRDRHGNSIENPHLLTLFH RLCKLLFFRIRPIFVFDGDA PLLKKQTLVKRRQRKDLASS DSRKTTEKLLKTFLKRQAIK TAFRSKRDEALPSLTQVRRE NDLYVLPPLQEEEKHSSEEE DEKEWQERMNQKQALQEEFF HNPQAIDIESEDFSSLPPEV KHEILTDMKEFTKRRRTLFE AMPEESDDFSQYQLKGLLK |
| Function | Single-stranded structure-specific DNA endonuclease involved in DNA excision repair. Makes the 3'incision in DNA nucleotide excision repair (NER). Binds and bends DNA repair bubble substrate and breaks base stacking at the single-strand/double-strand DNA junction of the DNA bubble. Plays a role in base excision repair (BER) by promoting the binding of DNA glycosylase NTHL1 to its substrate and increasing NTHL1 catalytic activity that removes oxidized pyrimidines from DNA. Involved in transcription-coupled nucleotide excision repair (TCR) which allows RNA polymerase II-blocking lesions to be rapidly removed from the transcribed strand of active genes. Functions during the initial step of TCR in cooperation with ERCC6/CSB to recognized stalled RNA polymerase II. Also, stimulates ERCC6/CSB binding to the DNA repair bubble and ERCC6/CSB ATPase activity. Required for DNA replication fork maintenance and preservation of genomic stability. Involved in homologous recombination repair (HRR) induced by DNA replication stress by recruiting RAD51, BRCA2, and PALB2 to the damaged DNA site. During HRR, binds to the replication fork with high specificity and stabilizes it. Also, acts upstream of HRR, to promote the release of BRCA1 from DNA. |
| Protein Name | Dna Excision Repair Protein Ercc-5Dna Repair Protein Complementing Xp-G CellsXeroderma Pigmentosum Group G-Complementing Protein |
| Database Links | Reactome: R-HSA-5696395Reactome: R-HSA-5696400Reactome: R-HSA-6782135 |
| Cellular Localisation | NucleusChromosomeColocalizes With Rad51 To Nuclear Foci In S PhaseLocalizes To Dna Double-Strand Breaks (Dbs) During Replication StressColocalizes With Brca2 To Nuclear Foci Following Dna Replication Stress |
| Alternative Antibody Names | Anti-Dna Excision Repair Protein Ercc-5 antibodyAnti-Dna Repair Protein Complementing Xp-G Cells antibodyAnti-Xeroderma Pigmentosum Group G-Complementing Protein antibodyAnti-ERCC5 antibodyAnti-ERCM2 antibodyAnti-XPG antibodyAnti-XPGC antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
