• Western blot analysis of 1) Hela, 2) HepG2, 3) 293T, 4) Jurkat, diluted at 1:2000. cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-ERCC1 antibody [1B10] (STJ96980)

SKU:
STJ96980

£46.00 - £226.00
Current Stock:
Host: Mouse
Applications: WB/IHC/IF
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-DNA excision repair protein ERCC-1 is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: 1B10
Conjugation: Unconjugated
Isotype: IgG1
Formulation: Liquid in PBS pH7.4, 0.5% BSA, 0.02% Sodium Azide and 50% Glycerol.
Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Dilution Range: IHC-P 100-300
WB 1:1000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ERCC1
Gene ID: 2067
Uniprot ID: ERCC1_HUMAN
Specificity: The antibody detects endogenous ERCC1 proteins.
Immunogen: Synthetic Peptide of ERCC1
Function Isoform 1: Non-catalytic component of a structure-specific DNA repair endonuclease responsible for the 5'-incision during DNA repair. Responsible, in conjunction with SLX4, for the first step in the repair of interstrand cross-links (ICL). Participates in the processing of anaphase bridge-generating DNA structures, which consist in incompletely processed DNA lesions arising during S or G2 phase, and can result in cytokinesis failure. Also required for homology-directed repair (HDR) of DNA double-strand breaks, in conjunction with SLX4. Isoform 2: Not functional in the nucleotide excision repair pathway. Isoform 3: Not functional in the nucleotide excision repair pathway. Isoform 4: Not functional in the nucleotide excision repair pathway.
Protein Name Dna Excision Repair Protein Ercc-1
Database Links Reactome: R-HSA-5685938
Reactome: R-HSA-5696395
Reactome: R-HSA-5696400
Reactome: R-HSA-6782135
Reactome: R-HSA-6783310
Cellular Localisation Isoform 1: Nucleus
Isoform 2: Cytoplasm
Nucleus
Isoform 3: Nucleus
Isoform 4: Nucleus
Alternative Antibody Names Anti-Dna Excision Repair Protein Ercc-1 antibody
Anti-ERCC1 antibody

Information sourced from Uniprot.org

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