Anti-EPOR antibody [Fs0378] (STJA0032015)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG2a
General Information
| Short Description | Mouse monoclonal anti-EPOR for use in ELISA and FCM in Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | ELISA/FCM |
| Host | Mouse |
| Reactivity | Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | Fs0378 |
| Isotype | IgG2a |
| Conjugation | Unconjugated |
| Purification | Protein A/G purified from cell culture supernatant |
| Formulation | 0.01M PBS, pH 7.4. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at-20°C 12 months. Store at-80°C long term. |
| Endotoxin | Please contact us for this information. |
Target Information
| Gene Symbol | Epor |
| Gene ID | 13857 |
| Uniprot ID | EPOR_MOUSE |
Additional Info
| Post Translational Modifications | The identity of the C-linked hexose on the WXXW motif has not been determined. It is probably mannose. On EPO stimulation, phosphorylated on C-terminal tyrosine residues by JAK2. The phosphotyrosine motifs are also recruitment sites for several SH2-containing proteins and adapter proteins which mediate cell proliferation. Phosphorylation on Tyr-453 is required for PTPN6 interaction, Tyr-425 for PTPN11. Tyr-425 is also required for SOCS3 binding, but Tyr-453/Tyr-455 motif is the preferred binding site. Ubiquitinated by the ECS(SOCS2) complex following ligand-binding and phosphorylation by JAK2, leading to its degradation by the proteasome. Regulation by the ECS(SOCS2) complex acts as a negative feedback loop of erythropoietin-mediated signaling pathway. Ubiquitination at Lys-280 mediates receptor internalization, whereas ubiquitination at Lys-452 promotes trafficking of activated receptors to the lysosomes for degradation. Ubiquitinated by NOSIP.appears to be either multi-monoubiquitinated or polyubiquitinated. Ubiquitination mediates proliferation and survival of EPO-dependent cells. |
| Function | Receptor for erythropoietin, which mediates erythropoietin-induced erythroblast proliferation and differentiation. Upon EPO stimulation, EPOR dimerizes triggering the JAK2/STAT5 signaling cascade. In some cell types, can also activate STAT1 and STAT3. May also activate the LYN tyrosine kinase. |
| Protein Name | Erythropoietin ReceptorEpo-R |
| Database Links | Reactome: R-MMU-9006335Reactome: -MMU-9027276Reactome: -MMU-9027284 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinIsoform Epor-F: Cell MembraneIsoform Epor-S: Secreted |
| Alternative Antibody Names | Anti-Erythropoietin Receptor antibodyAnti-Epo-R antibodyAnti-Epor antibody |
Information sourced from Uniprot.org