Anti-EPN1 antibody (130-180) (STJ119738)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal EPN1 (130-180) antibody for use in WB, IHC-P and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IHC-P:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | EPN1 |
| Gene ID | 29924 |
| Uniprot ID | EPN1_HUMAN |
| Immunogen Region | 130-180 |
| Immunogen Sequence | LVALLRDEDRLREERAHALK TKEKLAQTATASSAAVGSGP PPEAEQAWPQS |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 130-180 of human EPN1 (NP_037465.2). |
Additional Info
| Post Translational Modifications | Phosphorylated on serine and/or threonine residues in mitotic cells. Phosphorylation reduces interaction with REPS2, AP-2 and the membrane fraction. Depolarization of synaptosomes results in dephosphorylation. Ubiquitinated. |
| Function | Binds to membranes enriched in phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). Modifies membrane curvature and facilitates the formation of clathrin-coated invaginations. Regulates receptor-mediated endocytosis. |
| Protein Name | Epsin-1Eh Domain-Binding Mitotic PhosphoproteinEps-15-Interacting Protein 1 |
| Database Links | Reactome: R-HSA-182971Reactome: R-HSA-8856825Reactome: R-HSA-8856828 |
| Cellular Localisation | CytoplasmCell MembranePeripheral Membrane ProteinNucleusMembraneClathrin-Coated PitAssociated With The Cytoplasmic Membrane At Sites Where Clathrin-Coated Pits Are FormingColocalizes With Clathrin And Ap-2 In A Punctate Pattern On The Plasma MembraneDetected In Presynaptic Nerve Terminals And In Golgi StacksMay Shuttle To The Nucleus When Associated With Zbtb16/Znf145 |
| Alternative Antibody Names | Anti-Epsin-1 antibodyAnti-Eh Domain-Binding Mitotic Phosphoprotein antibodyAnti-Eps-15-Interacting Protein 1 antibodyAnti-EPN1 antibody |
Information sourced from Uniprot.org