• STJ73469 Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml) , showing cytoplasmic/Plasma Membrane and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).
  • STJ73469 Flow cytometric analysis of paraformaldehyde fixed HEK293 cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-EPI64/TBC1D10A antibody (N-Term) (STJ73469)

SKU:
STJ73469-100

£303.50
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Host: Goat
Applications: Pep-ELISA/IF/FC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-EPI64/TBC1D10A (N-Term) is suitable for use in ELISA, Immunofluorescence and Flow Cytometry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: IF-Strong expression of the protein seen in the cytoplasm/plasma membranes and nuclei of MCF7 cells. 10µg/ml
ELISA-antibody detection limit dilution 1:2000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: TBC1D10A
Gene ID: 83874
Uniprot ID: TB10A_HUMAN
Immunogen Region: N-Term
Accession Number: NP_114143; NP_001191169.1
Immunogen Sequence: AKSNGENGPRAPA
Function Acts as GTPase-activating protein for RAB27A, but not for RAB2A, RAB3A, nor RAB4A.
Protein Name Tbc1 Domain Family Member 10a
Ebp50-Pdx Interactor Of 64 Kda
Epi64 Protein
Rab27a-Gap-Alpha
Database Links Reactome: R-HSA-8854214
Cellular Localisation Cell Projection
Microvillus
Localizes To The Microvilli-Rich Region Of The Syncytiotrophoblast
In Melanocytes
Located At The Periphery Of Cells
Alternative Antibody Names Anti-Tbc1 Domain Family Member 10a antibody
Anti-Ebp50-Pdx Interactor Of 64 Kda antibody
Anti-Epi64 Protein antibody
Anti-Rab27a-Gap-Alpha antibody
Anti-TBC1D10A antibody
Anti-EPI64 antibody
Anti-TBC1D10 antibody

Information sourced from Uniprot.org

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