• Western blot analysis of extracts of various cell lines, using EPHB2 antibody (STJ111855) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of extracts of various cell lines, using EPHB2 antibody (STJ111855) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-EPHB2 antibody (250-540) (STJ111855)

SKU:
STJ111855

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-EPHB2 (250-540) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EPHB2
Gene ID: 2048
Uniprot ID: EPHB2_HUMAN
Immunogen Region: 250-540
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 250-540 of human EPHB2 (NP_059145.2).
Immunogen Sequence: PIGRCMCKAGFEAVENGTVC RGCPSGTFKANQGDEACTHC PINSRTTSEGATNCVCRNGY YRADLDPLDMPCTTIPSAPQ AVISSVNETSLMLEWTPPRD SGGREDLVYNIICKSCGSGR GACTRCGDNVQYAPRQLGLT EPRIYISDLLAHTQYTFEIQ AVNGVTDQSPFSPQFASVNI TTNQAAPSAVSIMHQVSRTV DSITLSWSQPDQPNGVILDY ELQYYEKELSEYNATAIKS
Tissue Specificity Brain, heart, lung, kidney, placenta, pancreas, liver and skeletal muscle. Preferentially expressed in fetal brain.
Post Translational Modifications Autophosphorylated.ligand binding stimulates autophosphorylation on tyrosine residues. Polyubiquitinated.ligand binding stimulates ubiquitination. Ubiquitinated by RNF186 at Lys-891, mainly through 'Lys-27'-linked polyubiquitin chains. Ligand binding induces cleavage by matrix metalloproteinases (MMPs) such as MMP7/MMP9, producing an EphB2/N-terminal fragment (NTF) and a C-terminal long fragment (EphB2-LF). EphB2-LF is further cleaved by MMPs, producing EphB2/CTF1 which is further cleaved by the PS1/gamma-secretase producing EphB2/CTF2.
Function Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Functions in axon guidance during development. Involved in the guidance of commissural axons, that form a major interhemispheric connection between the 2 temporal lobes of the cerebral cortex. Also involved in guidance of contralateral inner ear efferent growth cones at the midline and of retinal ganglion cell axons to the optic disk. In addition to axon guidance, also regulates dendritic spines development and maturation and stimulates the formation of excitatory synapses. Upon activation by EFNB1, abolishes the ARHGEF15-mediated negative regulation on excitatory synapse formation. Controls other aspects of development including angiogenesis, palate development and in inner ear development through regulation of endolymph production. Forward and reverse signaling through the EFNB2/EPHB2 complex regulate movement and adhesion of cells that tubularize the urethra and septate the cloaca. May function as a tumor suppressor. May be involved in the regulation of platelet activation and blood coagulation.
Protein Name Ephrin Type-B Receptor 2
Developmentally-Regulated Eph-Related Tyrosine Kinase
Elk-Related Tyrosine Kinase
Eph Tyrosine Kinase 3
Eph-Like Kinase 5
Ek5
Hek5
Renal Carcinoma Antigen Ny-Ren-47
Tyrosine-Protein Kinase Tyro5
Tyrosine-Protein Kinase Receptor Eph-3 Cleaved Into - Ephb2/Ctf1 - Ephb2/Ctf2
Database Links Reactome: R-HSA-2682334
Reactome: R-HSA-373760
Reactome: R-HSA-3928662
Reactome: R-HSA-3928664
Reactome: R-HSA-3928665
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Cell Projection
Axon
Dendrite
Alternative Antibody Names Anti-Ephrin Type-B Receptor 2 antibody
Anti-Developmentally-Regulated Eph-Related Tyrosine Kinase antibody
Anti-Elk-Related Tyrosine Kinase antibody
Anti-Eph Tyrosine Kinase 3 antibody
Anti-Eph-Like Kinase 5 antibody
Anti-Ek5 antibody
Anti-Hek5 antibody
Anti-Renal Carcinoma Antigen Ny-Ren-47 antibody
Anti-Tyrosine-Protein Kinase Tyro5 antibody
Anti-Tyrosine-Protein Kinase Receptor Eph-3 Cleaved Into - Ephb2/Ctf1 - Ephb2/Ctf2 antibody
Anti-EPHB2 antibody
Anti-DRT antibody
Anti-EPHT3 antibody
Anti-EPTH3 antibody
Anti-ERK antibody
Anti-HEK5 antibody
Anti-TYRO5 antibody

Information sourced from Uniprot.org

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