| Host: |
Mouse |
| Applications: |
WB |
| Reactivity: |
Chicken/Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Mouse monoclonal antibody anti-EphA4 (N-Term) is suitable for use in Western Blot research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
M280 |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG1 |
| Formulation: |
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Purification: |
Antigen Affinity Purified |
| Dilution Range: |
WB 1:1000IHCICCIP |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
EPHA4 |
| Gene ID: |
2043 |
| Uniprot ID: |
EPHA4_HUMAN |
| Immunogen Region: |
N-Term |
| Specificity: |
This antibody detects 95 and 120 kDa* bands corresponding to EphA4 in Western blots of HUVEC cells and a 120 kDa band in mouse brain tissue. |
| Immunogen: |
Clone M280 was generated from a recombinant protein corresponding to amino acids in the N-terminal region of human EphA4. This region has less than 50% homology to similar regions in other EphA and EphB family members. |
| Tissue Specificity | Ubiquitous. |
| Function | Receptor tyrosine kinase which binds membrane-bound ephrin family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Highly promiscuous, it has the unique property among Eph receptors to bind and to be physiologically activated by both GPI-anchored ephrin-A and transmembrane ephrin-B ligands including EFNA1 and EFNB3. Upon activation by ephrin ligands, modulates cell morphology and integrin-dependent cell adhesion through regulation of the Rac, Rap and Rho GTPases activity. Plays an important role in the development of the nervous system controlling different steps of axonal guidance including the establishment of the corticospinal projections. May also control the segregation of motor and sensory axons during neuromuscular circuit development. In addition to its role in axonal guidance plays a role in synaptic plasticity. Activated by EFNA1 phosphorylates CDK5 at 'Tyr-15' which in turn phosphorylates NGEF regulating RHOA and dendritic spine morphogenesis. In the nervous system, also plays a role in repair after injury preventing axonal regeneration and in angiogenesis playing a role in central nervous system vascular formation. Additionally, its promiscuity makes it available to participate in a variety of cell-cell signaling regulating for instance the development of the thymic epithelium. During development of the cochlear organ of Corti, regulates pillar cell separation by forming a ternary complex with ADAM10 and CADH1 which facilitates the cleavage of CADH1 by ADAM10 and disruption of adherens junctions. Phosphorylates CAPRIN1, promoting CAPRIN1-dependent formation of a membraneless compartment. |
| Protein Name | Ephrin Type-A Receptor 4Eph-Like Kinase 8Ek8Hek8Tyrosine-Protein Kinase Tyro1Tyrosine-Protein Kinase Receptor Sek |
| Database Links | Reactome: R-HSA-2682334Reactome: R-HSA-3928663Reactome: R-HSA-3928665Reactome: R-HSA-9824272 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinCell ProjectionAxonDendritePostsynaptic Density MembraneEarly EndosomeCell JunctionAdherens JunctionClustered Upon Activation And Targeted To Early Endosome |
| Alternative Antibody Names | Anti-Ephrin Type-A Receptor 4 antibodyAnti-Eph-Like Kinase 8 antibodyAnti-Ek8 antibodyAnti-Hek8 antibodyAnti-Tyrosine-Protein Kinase Tyro1 antibodyAnti-Tyrosine-Protein Kinase Receptor Sek antibodyAnti-EPHA4 antibodyAnti-HEK8 antibodyAnti-SEK antibodyAnti-TYRO1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
