• Western blot analysis of lysates from MCF-7 cells, using EPHA2/3/4 Antibody. The lane on the right is blocked with the synthesized peptide.
  • Western blot analysis of 3T3 cells using EphA2/3/4 Polyclonal Antibody
  • Immunofluorescence analysis of A549 cells, using EPHA2/3/4 Antibody. The picture on the right is blocked with the synthesized peptide.
  • Western blot analysis of various cells using EphA2/3/4 Polyclonal Antibody

Anti-EphA2/3/4 antibody (556-605 aa) (STJ92941)

SKU:
STJ92941

Current Stock:
Host: Rabbit
Applications: WB/IF/ELISA
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Ephrin type-A receptor 2 and Ephrin type-A receptor 3 and Ephrin type-A receptor 4 (556-605 aa) is suitable for use in Western Blot, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IF 1:200-1:1000
ELISA 1:40000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EPHA2
EPHA4
1969
2043
2042
EPHA2_HUMAN
EPHA4_HUMAN
EPHA3_HUMAN
Immunogen Region: 556-605 aa
Specificity: EphA2/3/4 Polyclonal Antibody detects endogenous levels of EphA2/3/4 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human EPHA2/3/4 at the amino acid range 556-605
Post Translational Modifications Autophosphorylates. Phosphorylated on tyrosine upon binding and activation by EFNA1. Phosphorylated residues Tyr-588 and Tyr-594 are required for binding VAV2 and VAV3 while phosphorylated residues Tyr-735 and Tyr-930 are required for binding PI3-kinase p85 subunit (PIK3R1, PIK3R2 or PIK3R3). These phosphorylated residues are critical for recruitment of VAV2 and VAV3 and PI3-kinase p85 subunit which transduce downstream signaling to activate RAC1 GTPase and cell migration. Dephosphorylation of Tyr-930 by PTPRF prevents the interaction of EPHA2 with NCK1. Phosphorylated at Ser-897 by PKB.serum-induced phosphorylation which targets EPHA2 to the cell leading edge and stimulates cell migration. Phosphorylation by PKB is inhibited by EFNA1-activated EPHA2 which regulates PKB activity via a reciprocal regulatory loop. Phosphorylated at Ser-897 in response to TNF by RPS6KA1 and RPS6KA3.RPS6KA-EPHA2 signaling pathway controls cell migration. Phosphorylated at Ser-897 by PKA.blocks cell retraction induced by EPHA2 kinase activity. Dephosphorylated by ACP1. Ubiquitinated by CHIP/STUB1. Ubiquitination is regulated by the HSP90 chaperone and regulates the receptor stability and activity through proteasomal degradation. ANKS1A prevents ubiquitination and degradation.
Function Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Activated by the ligand ephrin-A1/EFNA1 regulates migration, integrin-mediated adhesion, proliferation and differentiation of cells. Regulates cell adhesion and differentiation through DSG1/desmoglein-1 and inhibition of the ERK1/ERK2 (MAPK3/MAPK1, respectively) signaling pathway. May also participate in UV radiation-induced apoptosis and have a ligand-independent stimulatory effect on chemotactic cell migration. During development, may function in distinctive aspects of pattern formation and subsequently in development of several fetal tissues. Involved for instance in angiogenesis, in early hindbrain development and epithelial proliferation and branching morphogenesis during mammary gland development. Engaged by the ligand ephrin-A5/EFNA5 may regulate lens fiber cells shape and interactions and be important for lens transparency development and maintenance. With ephrin-A2/EFNA2 may play a role in bone remodeling through regulation of osteoclastogenesis and osteoblastogenesis. (Microbial infection) Acts as a receptor for hepatitis C virus (HCV) in hepatocytes and facilitates its cell entry. Mediates HCV entry by promoting the formation of the CD81-CLDN1 receptor complexes that are essential for HCV entry and by enhancing membrane fusion of cells expressing HCV envelope glycoproteins.
Protein Name Ephrin Type-A Receptor 2
Epithelial Cell Kinase
Tyrosine-Protein Kinase Receptor Eck
Database Links Reactome: R-HSA-2682334
Reactome: R-HSA-3928663
Reactome: R-HSA-3928665
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Cell Projection
Ruffle Membrane
Lamellipodium Membrane
Cell Junction
Focal Adhesion
Present At Regions Of Cell-Cell Contacts But Also At The Leading Edge Of Migrating Cells
Relocates From The Plasma Membrane To The Cytoplasmic And Perinuclear Regions In Cancer Cells
Alternative Antibody Names Anti-Ephrin Type-A Receptor 2 antibody
Anti-Epithelial Cell Kinase antibody
Anti-Tyrosine-Protein Kinase Receptor Eck antibody
Anti-EPHA2 antibody
Anti-ECK antibody

Information sourced from Uniprot.org

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