Anti-EPHA1 antibody [5D2] (STJ98022)

SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG1
ImmunogenPurified recombinant fragment of EphA1 expressed in E. Coli.
STJ98022
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General Information

Short DescriptionMouse monoclonal anti-Ephrin type-A receptor 1 for use in WB, IHC, IF and ELISA in Human samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/IHC/IF/ELISA
HostMouse
ReactivityHuman
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityMonoclonal
Clone ID5D2
IsotypeIgG1
ConjugationUnconjugated
Concentration1 mg/mL
PurificationAffinity purification
Dilution RangeWB 1:500-1:2000
IHC 1:200-1:1000
ELISA 1:10000
IF 1:50-200
FormulationLiquid in PBS containing 0.03% Sodium Azide.
Storage InstructionStore at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolEPHA1
Gene ID2041
Uniprot IDEPHA1_HUMAN
ImmunogenPurified recombinant fragment of EphA1 expressed in E. Coli.
SpecificityEphA1 Monoclonal Antibody detects endogenous levels of EphA1 protein.

Additional Info

Post Translational Modifications Phosphorylated. Autophosphorylation is stimulated by its ligand EFNA1. Ubiquitinated.
Function Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Binds with a low affinity EFNA3 and EFNA4 and with a high affinity to EFNA1 which most probably constitutes its cognate/functional ligand. Upon activation by EFNA1 induces cell attachment to the extracellular matrix inhibiting cell spreading and motility through regulation of ILK and downstream RHOA and RAC. Also plays a role in angiogenesis and regulates cell proliferation. May play a role in apoptosis.
Protein Name Ephrin Type-A Receptor 1
Hepha1
Eph Tyrosine Kinase
Eph Tyrosine Kinase 1
Erythropoietin-Producing Hepatoma Receptor
Tyrosine-Protein Kinase Receptor Eph
Database Links Reactome: R-HSA-2682334
Reactome: R-HSA-2892247
Reactome: R-HSA-3928663
Reactome: R-HSA-3928665
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-Ephrin Type-A Receptor 1 antibody
Anti-Hepha1 antibody
Anti-Eph Tyrosine Kinase antibody
Anti-Eph Tyrosine Kinase 1 antibody
Anti-Erythropoietin-Producing Hepatoma Receptor antibody
Anti-Tyrosine-Protein Kinase Receptor Eph antibody
Anti-EPHA1 antibody
Anti-EPH antibody
Anti-EPHT antibody
Anti-EPHT1 antibody

Information sourced from Uniprot.org

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