• Western blot analysis of extracts of various cell lines, using EPHA1 antibody (STJ29467) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.

Anti-EPHA1 antibody (1-230) (STJ29467)

SKU:
STJ29467

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-EPHA1 (1-230) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EPHA1
Gene ID: 2041
Uniprot ID: EPHA1_HUMAN
Immunogen Region: 1-230
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human EPHA1 (NP_005223.4).
Immunogen Sequence: MERRWPLGLGLVLLLCAPLP PGARAKEVTLMDTSKAQGEL GWLLDPPKDGWSEQQQILNG TPLYMYQDCPMQGRRDTDHW LRSNWIYRGEEASRVHVELQ FTVRDCKSFPGGAGPLGCKE TFNLLYMESDQDVGIQLRRP LFQKVTTVAADQSFTIRDLV SGSVKLNVERCSLGRLTRRG LYLAFHNPGACVALVSVRVF YQRCPETLNGLAQFPDTLPG PAGLVEVAGT
Tissue Specificity Overexpressed in several carcinomas.
Post Translational Modifications Phosphorylated. Autophosphorylation is stimulated by its ligand EFNA1. Ubiquitinated.
Function Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Binds with a low affinity EFNA3 and EFNA4 and with a high affinity to EFNA1 which most probably constitutes its cognate/functional ligand. Upon activation by EFNA1 induces cell attachment to the extracellular matrix inhibiting cell spreading and motility through regulation of ILK and downstream RHOA and RAC. Also plays a role in angiogenesis and regulates cell proliferation. May play a role in apoptosis.
Protein Name Ephrin Type-A Receptor 1
Hepha1
Eph Tyrosine Kinase
Eph Tyrosine Kinase 1
Erythropoietin-Producing Hepatoma Receptor
Tyrosine-Protein Kinase Receptor Eph
Database Links Reactome: R-HSA-2682334
Reactome: R-HSA-2892247
Reactome: R-HSA-3928663
Reactome: R-HSA-3928665
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-Ephrin Type-A Receptor 1 antibody
Anti-Hepha1 antibody
Anti-Eph Tyrosine Kinase antibody
Anti-Eph Tyrosine Kinase 1 antibody
Anti-Erythropoietin-Producing Hepatoma Receptor antibody
Anti-Tyrosine-Protein Kinase Receptor Eph antibody
Anti-EPHA1 antibody
Anti-EPH antibody
Anti-EPHT antibody
Anti-EPHT1 antibody

Information sourced from Uniprot.org

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