Anti-EPB41 antibody (700-800) (STJ11105556)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal Protein 4.1 (700-800) antibody for use in WB, IP and ELISA in human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IP/ELISA |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:1000-1:5000IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | EPB41 |
| Gene ID | 2035 |
| Uniprot ID | EPB41_HUMAN |
| Immunogen Region | 700-800 |
| Immunogen Sequence | RPSEWDKRLSTHSPFRTLNI NGQIPTGEGPPLVKTQTVTI SDNANAVKSEIPTKDVPIVH TETKTITYEAAQTDDNSGDL DPGVLLTAQTITSETPSSTT T |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 700-800 of human EPB41 (NP_001159477.1). |
Additional Info
| Post Translational Modifications | Phosphorylated at multiple sites by different protein kinases and each phosphorylation event selectively modulates the protein's functions. Phosphorylation on Tyr-660 reduces the ability of 4.1 to promote the assembly of the spectrin/actin/4.1 ternary complex. O-glycosylated.contains N-acetylglucosamine side chains in the C-terminal domain. |
| Function | Protein 4.1 is a major structural element of the erythrocyte membrane skeleton. It plays a key role in regulating membrane physical properties of mechanical stability and deformability by stabilizing spectrin-actin interaction. Recruits DLG1 to membranes. Required for dynein-dynactin complex and NUMA1 recruitment at the mitotic cell cortex during anaphase. |
| Protein Name | Protein 4.1P4.14.1rBand 4.1Epb4.1Erythrocyte Membrane Protein Band 4.1 |
| Database Links | Reactome: R-HSA-6794361 |
| Cellular Localisation | CytoplasmCytoskeletonCell CortexNucleus |
| Alternative Antibody Names | Anti-Protein 4.1 antibodyAnti-P4.1 antibodyAnti-4.1r antibodyAnti-Band 4.1 antibodyAnti-Epb4.1 antibodyAnti-Erythrocyte Membrane Protein Band 4.1 antibodyAnti-EPB41 antibodyAnti-E41P antibody |
Information sourced from Uniprot.org