• Western blot analysis of various lysates using AER61 Rabbit monoclonal antibody (STJ11104214) at1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
  • Western blot analysis of various lysates using AER61 Rabbit monoclonal antibody (STJ11104214) at1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-EOGT antibody (1-150) [S4214RM] (STJ11104214)

SKU:
STJ11104214

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Egf Domain-Specific O-Linked N-Acetylglucosamine Transferase (1-150) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S4214RM
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EOGT
Gene ID: 285203
Uniprot ID: EOGT_HUMAN
Immunogen Region: 1-150
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human AER61 (NP_775925.1).
Immunogen Sequence: MLMLFVFGVLLHEVSLSGQN EAPPNTHSIPGEPLYNYASI RLPEEHIPFFLHNNRHIATV CRKDSLCPYKKHLEKLKYCW GYEKSCKPEFRFGYPVCSYV DMGWTDTLESAEDIFWKQAD FGYARERLEEMHVLCQPKET SDSSLVCSRY
Function Catalyzes the transfer of a single N-acetylglucosamine from UDP-GlcNAc to a serine or threonine residue in extracellular proteins resulting in their modification with a beta-linked N-acetylglucosamine (O-GlcNAc). Specifically glycosylates the Thr residue located between the fifth and sixth conserved cysteines of folded EGF-like domains.
Protein Name Egf Domain-Specific O-Linked N-Acetylglucosamine Transferase
Extracellular O-Linked N-Acetylglucosamine Transferase
Cellular Localisation Endoplasmic Reticulum Lumen
Alternative Antibody Names Anti-Egf Domain-Specific O-Linked N-Acetylglucosamine Transferase antibody
Anti-Extracellular O-Linked N-Acetylglucosamine Transferase antibody
Anti-EOGT antibody
Anti-AER61 antibody
Anti-C3orf64 antibody
Anti-EOGT1 antibody

Information sourced from Uniprot.org

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