• Immunohistochemistry analysis of ENTPD6 in paraffin-embedded mouse spinal cord using ENTPD6 Rabbit polyclonal antibody (STJ118101) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of ENTPD6 in paraffin-embedded human colon carcinoma using ENTPD6 Rabbit polyclonal antibody (STJ118101) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of ENTPD6 in paraffin-embedded rat brain using ENTPD6 Rabbit polyclonal antibody (STJ118101) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using ENTPD6 Rabbit polyclonal antibody (STJ118101) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ENTPD6 antibody (61-300) (STJ118101)

SKU:
STJ118101

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ENTPD6 (61-300) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:100-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ENTPD6
Gene ID: 955
Uniprot ID: ENTP6_HUMAN
Immunogen Region: 61-300
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 61-300 of human ENTPD6 (NP_001304870.1).
Immunogen Sequence: WHRATATQAFFSITRAAPGA RWGQQAHSPLGTAADGHEVF YGIMFDAGSTGTRVHVFQFT RPPRETPTLTHETFKALKPG LSAYADDVEKSAQGIRELLD VAKQDIPFDFWKATPLVLKA TAGLRLLPGEKAQKLLQKVK EVFKASPFLVGDDCVSIMNG TDEGVSAWITINFLTGSLKT PGGSSVGMLDLGGGSTQIAF LPRVEGTLQASPPGYLTALR MFNRTYKLYSYSYLGLGLM
Tissue Specificity Expressed in most tissues, but predominantly in heart.
Post Translational Modifications The secreted form may be produced by intracellular processing. N-glycosylated.
Function Catalyzes the hydrolysis of nucleoside triphosphates and diphosphates in a calcium- or magnesium-dependent manner. Has a strong preference for nucleoside diphosphates, preferentially hydrolyzes GDP, IDP, and UDP, with slower hydrolysis of CDP, ITP, GTP, CTP, ADP, and UTP and virtually no hydrolysis of ATP. The membrane bound form might support glycosylation reactions in the Golgi apparatus and, when released from cells, might catalyze the hydrolysis of extracellular nucleotides.
Protein Name Ectonucleoside Triphosphate Diphosphohydrolase 6
Ntpdase 6
Cd39 Antigen-Like 2
Database Links Reactome: R-HSA-8850843
Cellular Localisation Golgi Apparatus Membrane
Single-Pass Type Ii Membrane Protein
Secreted
Cell Membrane
Exists As A Secreted And Membrane-Bound Forms In The Medium Of Transfected Cells
The Secreted Form Is Predominant
Alternative Antibody Names Anti-Ectonucleoside Triphosphate Diphosphohydrolase 6 antibody
Anti-Ntpdase 6 antibody
Anti-Cd39 Antigen-Like 2 antibody
Anti-ENTPD6 antibody
Anti-CD39L2 antibody
Anti-IL6ST2 antibody

Information sourced from Uniprot.org

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