• Immunohistochemistry analysis of ENTPD5 in paraffin-embedded human small intestine tissue using ENTPD5 Rabbit monoclonal antibody (STJ11103069) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of ENTPD5 in paraffin-embedded mouse kidney tissue using ENTPD5 Rabbit monoclonal antibody (STJ11103069) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of ENTPD5 in paraffin-embedded rat liver tissue using ENTPD5 Rabbit monoclonal antibody (STJ11103069) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of ENTPD5 in paraffin-embedded rat kidney tissue using ENTPD5 Rabbit monoclonal antibody (STJ11103069) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Western blot analysis of various lysates using ENTPD5 Rabbit monoclonal antibody (STJ11103069) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Western blot analysis of various lysates using ENTPD5 Rabbit monoclonal antibody (STJ11103069) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-ENTPD5 antibody (100-200) [S9MR] (STJ11103069)

SKU:
STJ11103069

£212.50 - £501.50
Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-ENTPD5 (100-200) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S9MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:3000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ENTPD5
Gene ID: 957
Uniprot ID: ENTP5_HUMAN
Immunogen Region: 100-200
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 100-200 of human ENTPD5 (O75356).
Immunogen Sequence: GAETVQGLLEVAKDSIPRSH WKKTPVVLKATAGLRLLPEH KAKALLFEVKEIFRKSPFLV PKGSVSIMDGSDEGILAWVT VNFLTGQLHGHRQETVGTLD L
Tissue Specificity Expressed in adult liver, kidney, prostate, testis and colon. Much weaker expression in other tissues.
Post Translational Modifications N-glycosylated.high-mannose type. Glycosylation is not essential for enzymatic activity.
Function Hydrolyzes nucleoside diphosphates with a preference for GDP, IDP and UDP compared to ADP and CDP. In the lumen of the endoplasmic reticulum, hydrolyzes UDP that acts as an end-product feedback inhibitor of the UDP-Glc:glycoprotein glucosyltransferases. UMP can be transported back by an UDP-sugar antiporter to the cytosol where it is consumed to regenerate UDP-glucose. Therefore, it positively regulates protein reglucosylation by clearing UDP from the ER lumen and by promoting the regeneration of UDP-glucose. Protein reglucosylation is essential to proper glycoprotein folding and quality control in the ER.
Protein Name Nucleoside Diphosphate Phosphatase Entpd5
Cd39 Antigen-Like 4
Er-Udpase
Ectonucleoside Triphosphate Diphosphohydrolase 5
Ntpdase 5
Guanosine-Diphosphatase Entpd5
Gdpase Entpd5
Inosine Diphosphate Phosphatase Entpd5
Nucleoside Diphosphatase
Uridine-Diphosphatase Entpd5
Udpase Entpd5
Database Links Reactome: R-HSA-8850843
Reactome: R-HSA-9660826
Cellular Localisation Endoplasmic Reticulum
Secreted
Alternative Antibody Names Anti-Nucleoside Diphosphate Phosphatase Entpd5 antibody
Anti-Cd39 Antigen-Like 4 antibody
Anti-Er-Udpase antibody
Anti-Ectonucleoside Triphosphate Diphosphohydrolase 5 antibody
Anti-Ntpdase 5 antibody
Anti-Guanosine-Diphosphatase Entpd5 antibody
Anti-Gdpase Entpd5 antibody
Anti-Inosine Diphosphate Phosphatase Entpd5 antibody
Anti-Nucleoside Diphosphatase antibody
Anti-Uridine-Diphosphatase Entpd5 antibody
Anti-Udpase Entpd5 antibody
Anti-ENTPD5 antibody
Anti-CD39L4 antibody
Anti-PCPH antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance