Anti-Enpp1 antibody (580-730 aa) (STJ11106415)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal Enpp1 (580-730 aa) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | Enpp1 |
| Gene ID | 18605 |
| Uniprot ID | ENPP1_MOUSE |
| Immunogen Region | 580-730 aa |
| Immunogen Sequence | PIYNPSHPKEEGFLSQCPIK STSNDLGCTCDPWIVPIKDF EKQLNLTTEDDDIYHMTVPY GRPRILLKQHRVCLLQQQQF LTGYSLDLLMPLWASYTFLS NDQFSRDDFSNCLYQDLRIP LSPVHKCSYYKSNSKLSYGF LTPPRLNRVSN |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 580-730 of mouse Enpp1 (NP_032839.3). |
Additional Info
| Tissue Specificity | Selectively expressed on the surface of antibody-secreting cells. Expressed in osteocytes and osteoclasts. |
| Post Translational Modifications | N-glycosylated. The secreted form is produced through cleavage at Lys-85 by intracellular processing. |
| Function | Nucleotide pyrophosphatase that generates diphosphate (PPi) and functions in bone mineralization and soft tissue calcification by regulating pyrophosphate levels. PPi inhibits bone mineralization and soft tissue calcification by binding to nascent hydroxyapatite crystals, thereby preventing further growth of these crystals. Preferentially hydrolyzes ATP, but can also hydrolyze other nucleoside 5' triphosphates such as GTP, CTP and UTP to their corresponding monophosphates with release of pyrophosphate, as well as diadenosine polyphosphates, and also 3',5'-cAMP to AMP. May also be involved in the regulation of the availability of nucleotide sugars in the endoplasmic reticulum and Golgi, and the regulation of purinergic signaling. Inhibits ectopic joint calcification and maintains articular chondrocytes by repressing hedgehog signaling.it is however unclear whether hedgehog inhibition is direct or indirect. Appears to modulate insulin sensitivity. Also involved in melanogenesis. Also able to hydrolyze 2',3'-cGAMP (cyclic GMP-AMP), a second messenger that activates TMEM173/STING and triggers type-I interferon production. 2',3'-cGAMP degradation takes place in the lumen or extracellular space, and not in the cytosol where it is produced.the role of 2',3'-cGAMP hydrolysis is therefore unclear. Not able to hydrolyze the 2',3'-cGAMP linkage isomer 3',3'-cGAMP. |
| Protein Name | Ectonucleotide Pyrophosphatase/Phosphodiesterase Family Member 1E-Npp 1Alkaline Phosphodiesterase ILymphocyte Antigen 41Ly-41Nucleotide DiphosphataseNucleotide PyrophosphataseNppasePhosphodiesterase I/Nucleotide Pyrophosphatase 1Plasma-Cell Membrane Glycoprotein Pc-1 Cleaved Into - Ectonucleotide Pyrophosphatase/Phosphodiesterase Family Member 1 - Secreted Form |
| Database Links | Reactome: R-MMU-196843 |
| Cellular Localisation | Ectonucleotide Pyrophosphatase/Phosphodiesterase Family Member 1: Cell MembraneSingle-Pass Type Ii Membrane ProteinBasolateral Cell MembraneTargeted To The Basolateral Membrane In Polarized Epithelial Cells And In HepatocytesAnd To Matrix Vesicles In OsteoblastsEctonucleotide Pyrophosphatase/Phosphodiesterase Family Member 1Secreted Form: SecretedSecreted Following Proteolytic Cleavage |
| Alternative Antibody Names | Anti-Ectonucleotide Pyrophosphatase/Phosphodiesterase Family Member 1 antibodyAnti-E-Npp 1 antibodyAnti-Alkaline Phosphodiesterase I antibodyAnti-Lymphocyte Antigen 41 antibodyAnti-Ly-41 antibodyAnti-Nucleotide Diphosphatase antibodyAnti-Nucleotide Pyrophosphatase antibodyAnti-Nppase antibodyAnti-Phosphodiesterase I/Nucleotide Pyrophosphatase 1 antibodyAnti-Plasma-Cell Membrane Glycoprotein Pc-1 Cleaved Into - Ectonucleotide Pyrophosphatase/Phosphodiesterase Family Member 1 - Secreted Form antibodyAnti-Enpp1 antibodyAnti-Npps antibodyAnti-Pc1 antibodyAnti-Pdnp1 antibody |
Information sourced from Uniprot.org