• Immunohistochemistry analysis of paraffin-embedded human stomach using ENPEP antibody (STJ28985) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human prostate using ENPEP antibody (STJ28985) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat kidney using ENPEP antibody (STJ28985) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of mouse lung, using ENPEP antibody (STJ28985) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-ENPEP antibody (708-957) (STJ28985)

SKU:
STJ28985

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ENPEP (708-957) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ENPEP
Gene ID: 2028
Uniprot ID: AMPE_HUMAN
Immunogen Region: 708-957
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 708-957 of human ENPEP (NP_001968.3).
Immunogen Sequence: DDKELYPMIEEYFQGQVKPI ADSLGWNDAGDHVTKLLRSS VLGFACKMGDREALNNASSL FEQWLNGTVSLPVNLRLLVY RYGMQNSGNEISWNYTLEQY QKTSLAQEKEKLLYGLASVK NVTLLSRYLDLLKDTNLIKT QDVFTVIRYISYNSYGKNMA WNWIQLNWDYLVNRYTLNNR NLGRIVTIAEPFNTELQLWQ MESFFAKYPQAGAGEKPREQ VLETVKNNIEWLKQHRNTI
Tissue Specificity Expressed in choriocarcinoma cancer cell lines (at protein level). Expressed by epithelial cells of the proximal tubule cells and the glomerulus of the nephron. Also found in a variety of other tissues.
Function Regulates central hypertension through its calcium-modulated preference to cleave N-terminal acidic residues from peptides such as angiotensin II.
Protein Name Glutamyl Aminopeptidase
Eap
Aminopeptidase A
Ap-A
Differentiation Antigen Gp160
Cd Antigen Cd249
Database Links Reactome: R-HSA-2022377
Cellular Localisation Cell Membrane
Single-Pass Type Ii Membrane Protein
Alternative Antibody Names Anti-Glutamyl Aminopeptidase antibody
Anti-Eap antibody
Anti-Aminopeptidase A antibody
Anti-Ap-A antibody
Anti-Differentiation Antigen Gp160 antibody
Anti-Cd Antigen Cd249 antibody
Anti-ENPEP antibody

Information sourced from Uniprot.org

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