Anti-eNOS antibody [2E8-5A5-7B1] (STJA0032818)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG1
ImmunogenRecombinant Protein of eNOS
General Information
| Short Description | Mouse monoclonal anti-eNOS for use in WB in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB |
| Host | Mouse |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | 2E8-5A5-7B1 |
| Isotype | IgG1 |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | WB 1:500-1:1000 |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | NOS3 |
| Gene ID | 4846 |
| Uniprot ID | NOS3_HUMAN |
| Immunogen | Recombinant Protein of eNOS |
Additional Info
| Tissue Specificity | Platelets, placenta, liver and kidney. |
| Post Translational Modifications | Phosphorylation by AMPK at Ser-1177 in the presence of Ca(2+)-calmodulin (CaM) activates activity. In absence of Ca(2+)-calmodulin, AMPK also phosphorylates Thr-495, resulting in inhibition of activity. Phosphorylation of Ser-114 by CDK5 reduces activity. |
| Function | Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. Isoform eNOS13C: Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1. |
| Protein Name | Nitric Oxide Synthase 3Constitutive NosCnosEc-NosNos Type IiiNosiiiNitric Oxide Synthase - EndothelialEndothelial NosEnos |
| Database Links | Reactome: R-HSA-1222556Reactome: R-HSA-1474151Reactome: R-HSA-203615Reactome: R-HSA-203641Reactome: R-HSA-203754Reactome: R-HSA-392154Reactome: R-HSA-5218920Reactome: R-HSA-9009391Reactome: R-HSA-9856530 |
| Cellular Localisation | Cell MembraneMembraneCaveolaCytoplasmCytoskeletonGolgi ApparatusSpecifically Associates With Actin Cytoskeleton In The G2 Phase Of The Cell CycleWhich Is Favored By Interaction With Nosip And Results In A Reduced Enzymatic Activity |
| Alternative Antibody Names | Anti-Nitric Oxide Synthase 3 antibodyAnti-Constitutive Nos antibodyAnti-Cnos antibodyAnti-Ec-Nos antibodyAnti-Nos Type Iii antibodyAnti-Nosiii antibodyAnti-Nitric Oxide Synthase - Endothelial antibodyAnti-Endothelial Nos antibodyAnti-Enos antibodyAnti-NOS3 antibody |
Information sourced from Uniprot.org